Instruments by Science Group
Macromolecular
Soft Condensed 
Imaging and
Biological
Magnetic
Structures and
Crystallography 
Spectroscopy I24 Contact
Beamline Phone Number:
+44 (0) 1235 778950
Principal Beamline Scientist:
Robin Owen
Tel: +44 (0) 1235 778522
E-mail: [email protected]
Science Group Leader
Dave Hall
Email: [email protected]
Tel: +44 (0) 1235 778926
I24 Microfocus MX
Status: Operational
Beamsize: 5x5 - 50x40 (variable aspect ratio)
Detector: Pilatus3 6M
Wavelength: 0.62Å - 1.77Å
Detector: Pilatus3 6M
Wavelength: 0.62Å - 1.77Å
These publications are based on data gathered on beamline I24. If you are aware of any errors or publications that should be listed here, please send details to [email protected].
66 items found for 2025 [total: 1243]
On the mechanism of K+ transport through the inter-subunit tunnel of KdpFABC Journal Of General Physiology, 158 Dec 2025 DOI: 10.1085/jgp.202513794
Designing novel solenoid proteins with in silico evolution Communications Chemistry, 35 Dec 2025 DOI: 10.1038/s42004-025-01817-3
A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system Acta Crystallographica Section D, 81 Dec 2025 DOI: 10.1107/S2059798325008782
Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
A global survey of intramolecular isopeptide bonds Protein Science, 34 Dec 2025 DOI: 10.1002/pro.70342
Space-filling efficiency and optical properties of hemoglycin Monthly Notices Of The Royal Astronomical Society, 543 Nov 2025 DOI: 10.1093/mnras/staf1484
Journal Paper B22-Multimode InfraRed imaging And Microspectroscopy I11-High Resolution Powder Diffraction I24-Microfocus Macromolecular Crystallography open access
State-selective small molecule degraders that preferentially remove aggregates and oligomers Nature Communications, 16 Nov 2025 DOI: 10.1038/s41467-025-65454-z
Journal Paper I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Preclinical assessment of two FcγRI-specific antibodies that competitively inhibit immune complex-FcγRI binding to suppress autoimmune responses Nature Communications, 16 Nov 2025 DOI: 10.1038/s41467-025-65133-z
Droplet microfluidic and microcrystal strategies for time-resolved serial crystallography of enzymes at synchrotron and XFEL sources Nov 2025
Thesis I24-Microfocus Macromolecular Crystallography
Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes Iscience, Nov 2025 DOI: 10.1016/j.isci.2025.113926
An asymmetric hydrogen atom transferase with an abiological thiophenol cofactor Journal Of The American Chemical Society, Nov 2025 DOI: 10.1021/jacs.5c12516
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography
The structure and function of extracellular redox enzymes Nov 2025
Thesis B21-High Throughput SAXS I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography
Gluebodies offer a route to improve crystal reliability and diversity through transferable nanobody mutations that introduce constitutive close contacts Acs Central Science, Oct 2025 DOI: 10.1021/acscentsci.5c00937
Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Identification of a highly cooperative PROTAC degrader targeting GTP-loaded KRAS(On) alleles Journal Of The American Chemical Society, Oct 2025 DOI: 10.1021/jacs.5c10354
Penetrant PKCb mutation in ATLL displays a mixed gain-of-function Biochemical Journal, Oct 2025 DOI: 10.1042/BCJ20253384
Climbing mountains: building a data capture and correction system for JUNGFRAU 9M Oct 2025 DOI: 10.1063/4.0000827
Dimethoxybenzohomoadamantane-based soluble epoxide hydrolase inhibitors: in vivo efficacy in a murine model of chemotherapy-induced neuropathic pain Journal Of Enzyme Inhibition And Medicinal Chemistry, Oct 2025 DOI: 10.1080/14756366.2025.2574990
Probing the molecular determinants of Ty1 retrotransposon restriction specificity in yeast Plos Genetics, 21 Oct 2025 DOI: 10.1371/journal.pgen.1011898
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Exploiting the cryptic αd pocket of casein kinase 2α (CK2α) to deliver highly potent and selective Type 1 inhibitors Journal Of Medicinal Chemistry, Oct 2025 DOI: 10.1021/acs.jmedchem.5c01807
Journal Paper I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Conformational dynamics of the bacterial E3 ligase SspH1 Journal Of Biological Chemistry, 169 Sept 2025 DOI: 10.1016/j.jbc.2025.110671
Frustration in the protein-protein interface plays a central role in the cooperativity of PROTAC ternary complexes Nature Communications, 16 Sept 2025 DOI: 10.1038/s41467-025-63713-7
Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide Iucrj, 12 Sept 2025 DOI: 10.1107/S2052252525006645
Mechanism and cellular actions of the potent AMPK inhibitor BAY-3827 Science Advances, 11 Aug 2025 DOI: 10.1126/sciadv.adx2434
Journal Paper I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
NitrOFF: An engineered fluorescent biosensor to illuminate nitrate transport in living cells Angewandte Chemie International Edition, 135 Aug 2025 DOI: 10.1002/anie.202508058
Journal Paper I24-Microfocus Macromolecular Crystallography
Chemoenzymatic total synthesis of the antibiotic (−)-13-deoxytetrodecamycin using the Diels–Alderase TedJ Chemical Science, 48 Aug 2025 DOI: 10.1039/D5SC05480J
Double crossed? Structural and computational studies of an unusually crosslinked haem in Methylococcus capsulatus cytochrome P460 Chemical Science, Aug 2025 DOI: 10.1039/D5SC04213E
Journal Paper I24-Microfocus Macromolecular Crystallography VMXi-Versatile Macromolecular Crystallography in situ open access
De novo designed 3-helix bundle peptides and proteins with controlled topology and stability Chemical Science, Aug 2025 DOI: 10.1039/D5SC05576H
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Extracellular catalysis of environmental substrates by Shewanella oneidensis MR ‐1 occurs via active sites on the C‐terminal domains of MtrC Protein Science, 34 Aug 2025 DOI: 10.1002/pro.70243
Electrostatic interactions influence diazabicyclooctane inhibitor potency against OXA-48-like β-lactamases Rsc Medicinal Chemistry, Aug 2025 DOI: 10.1039/D5MD00512D
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Conformational dynamics and membrane insertion mechanism of B4GALNT1 in ganglioside synthesis Nature Communications, 16 Jul 2025 DOI: 10.1038/s41467-025-60593-9
Journal Paper I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Molecular basis of Fab-dependent IgA antibody recognition by gut-bacterial metallopeptidases The Embo Journal, 630 Jul 2025 DOI: 10.1038/s44318-025-00518-w
Identification of nanomolar adenosine A2A receptor ligands using reinforcement learning and structure-based drug design Nature Communications, 16 Jul 2025 DOI: 10.1038/s41467-025-60629-0
Structure-guided mechanistic investigation of stereochemical inversion during the octose formation catalyzed by an atypical transaldolase AprG in the biosynthesis of apramycin Acs Catalysis, Jul 2025 DOI: 10.1021/acscatal.5c03420
Journal Paper I24-Microfocus Macromolecular Crystallography
Structure-guided phage display discovery of antibodies for (S)Tn-glycans in protein context Nature Chemical Biology, 13 Jul 2025 DOI: 10.1038/s41589-025-01971-8
Journal Paper I24-Microfocus Macromolecular Crystallography
Macrocyclization of backbone N-methylated peptides by a prolyl oligopeptidase with a distinctive substrate recognition mechanism Chemical Science, 57 Jul 2025 DOI: 10.1039/D5SC03723A
Journal Paper I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Fragment-based development of small molecule inhibitors targeting Mycobacterium tuberculosis cholesterol metabolism Journal Of Medicinal Chemistry, Jul 2025 DOI: 10.1021/acs.jmedchem.5c00478
Journal Paper I02-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Mechanism and kinetics of turnover inhibitors of nicotinamide N-methyl transferase in vitro and in vivo Journal Of Biological Chemistry, 301 June 2025 DOI: 10.1016/j.jbc.2025.108492
Structure and stabilization of the antigenic glycoprotein building blocks of the New World mammarenavirus spike complex Mbio, June 2025 DOI: 10.1128/mbio.01076-25
Journal Paper I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Understanding the substrate recognition and catalytic mechanism of methyl fucosidases from glycoside hydrolase family 139 Journal Of Biological Chemistry, 11 June 2025 DOI: 10.1016/j.jbc.2025.110407
Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations Acta Crystallographica Section D Structural Biology, 81 June 2025 DOI: 10.1107/S2059798325004589
Journal Paper I24-Microfocus Macromolecular Crystallography VMXi-Versatile Macromolecular Crystallography in situ open access
Botulinum neurotoxin A mutants with enhanced ganglioside binding show improved potency and altered ganglioside selectivity Communications Chemistry, 8 June 2025 DOI: 10.1038/s42004-025-01569-0
HATRX for radiation damage study June 2025
Conference Paper I24-Microfocus Macromolecular Crystallography
Fragment-based drug discovery for transthyretin kinetic stabilisers using a novel capillary zone electrophoresis method Plos One, 20 May 2025 DOI: 10.1371/journal.pone.0323816
Journal Paper B23-Circular Dichroism I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Cyanophenylalanine as an infrared probe for iron-sulfur cluster redox state in multicenter metalloenzymes Chembiochem, 2 May 2025 DOI: 10.1002/cbic.202500251
Journal Paper I03-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
A combined methodology for radiation damage mitigation and enzyme intermediate study in macromolecular crystals May 2025 DOI: 10.25392/leicester.data.29155316.v1
Thesis I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography VMXm-Versatile Macromolecular Crystallography microfocus
Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base Nature Communications, 16 May 2025 DOI: 10.1038/s41467-025-60250-1
Design of novel mercapto-3-phenylpropanoyl dipeptides as dual angiotensin-converting enzyme C–domain-selective/neprilysin inhibitors Journal Of Medicinal Chemistry, Apr 2025 DOI: 10.1021/acs.jmedchem.5c00329
Journal Paper I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Structure and catalytic mechanism of methylisocitrate lyase, a potential drug target against Coxiella burnetii Journal Of Biological Chemistry, 42 Apr 2025 DOI: 10.1016/j.jbc.2025.108517
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Substitutions at the C-8 position of quinazolin-4-ones improve the potency of nicotinamide site binding tankyrase inhibitors European Journal Of Medicinal Chemistry, 288 Apr 2025 DOI: 10.1016/j.ejmech.2025.117397
Microcrystallization and room-temperature serial crystallography structure of human cytochrome P450 3A4 Archives Of Biochemistry And Biophysics, 28 Apr 2025 DOI: 10.1016/j.abb.2025.110419
Journal Paper I24-Microfocus Macromolecular Crystallography
Comparative analysis of STP6 and STP10 unravels molecular selectivity in sugar transport proteins Proceedings Of The National Academy Of Sciences, 122 Apr 2025 DOI: 10.1073/pnas.2417370122
Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development Elife, Apr 2025 DOI: 10.7554/eLife.105317
De novo design of parallel and antiparallel A3B3heterohexameric α-helical barrels Biochemistry, Apr 2025 DOI: 10.1021/acs.biochem.4c00584
Structure and unusual binding mechanism of the hyaluronan receptor LYVE-1 mediating leucocyte entry to lymphatics Nature Communications, 16 Mar 2025 DOI: 10.1038/s41467-025-57866-8
Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Structural plasticity of Plasmodium falciparum plasmepsin X to accommodate binding of potent macrocyclic hydroxyethylamine inhibitors Journal Of Molecular Biology, 16 Mar 2025 DOI: 10.1016/j.jmb.2025.169062
Structure of the outer membrane transporter fema and its role in the uptake of ferric dihydro-aeruginoic acid and ferric aeruginoic acid in Pseudomonas aeruginosa Acs Chemical Biology, Mar 2025 DOI: 10.1021/acschembio.4c00820
Solid-state NMR of membrane peptides and proteins in the lipid cubic phase Biophysical Journal, Mar 2025 DOI: 10.1016/j.bpj.2025.03.012
The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly Science Advances, 11 Mar 2025 DOI: 10.1126/sciadv.adr5744
Journal Paper I04-1-Macromolecular Crystallography (fixed wavelength) I24-Microfocus Macromolecular Crystallography open access
Sequence rules for a long SPOP-binding degron required for protein ubiquitylation Biochemical Journal, Mar 2025 DOI: 10.1042/BCJ20253041
Journal Paper I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Crystal structure of ferric recombinant horseradish peroxidase Jbic Journal Of Biological Inorganic Chemistry, 2 Mar 2025 DOI: 10.1007/s00775-025-02103-2
Journal Paper I03-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Reversible small molecule multivariant ras inhibitors display tunable affinity for the active and inactive forms of Ras Journal Of Medicinal Chemistry, 49 Mar 2025 DOI: 10.1021/acs.jmedchem.4c02929
Journal Paper I03-Macromolecular Crystallography I04-1-Macromolecular Crystallography (fixed wavelength) I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Bacterial polysaccharide lyase family 33: Specificity from an evolutionarily conserved binding tunnel Proceedings Of The National Academy Of Sciences, 122 Feb 2025 DOI: 10.1073/pnas.2421623122
Journal Paper B21-High Throughput SAXS I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Virtual fragment screening for DNA repair inhibitors in vast chemical space Nature Communications, 16 Feb 2025 DOI: 10.1038/s41467-025-56893-9
Journal Paper I03-Macromolecular Crystallography I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography open access
Structural enzymology of spirotetronate cyclases Feb 2025
Thesis I04-Macromolecular Crystallography I24-Microfocus Macromolecular Crystallography
Trojan horse peptide conjugates remodel the activity spectrum of clinical antibiotics Proceedings Of The National Academy Of Sciences, 122 Jan 2025 DOI: 10.1073/pnas.2319483121
Journal Paper I24-Microfocus Macromolecular Crystallography
Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide Open Biology, 15 Jan 2025 DOI: 10.1098/rsob.240310
Journal Paper I03-Macromolecular Crystallography I23-Long wavelength MX I24-Microfocus Macromolecular Crystallography Krios II-Titan Krios II at Diamond open access
Functional characterization of the polymerase domain of polymerase θ in humans and T. cruzi Jan 2025
Thesis I24-Microfocus Macromolecular Crystallography
- Hridya Valia Madapally, Adel Hussein, Martin Wazar Eriksen, Bjorn Panyella Pedersen, David L. Stokes, Himanshu Khandelia, "On the mechanism of K+ transport through the inter-subunit tunnel of KdpFABC", Journal Of General Physiology, 158 (2025), DOI: 10.1085/jgp.202513794
- Daniella Pretorius, Georgi I. Nikov, Kono Washio, Steve-William Florent, Henry N. Taunt, Sergey Ovchinnikov, James W. Murray, "Designing novel solenoid proteins with in silico evolution", Communications Chemistry, 35 (2025), DOI: 10.1038/s42004-025-01817-3
- Ryan Griffiths, Hans Pfalzgraf, Dimitris Latousakis, Gareth Ashworth, Changjiang Dong, Andrew Hemmings, Nathalie Juge, "A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system", Acta Crystallographica Section D, 81 (2025), DOI: 10.1107/S2059798325008782
- Francesca Coscia, Ioannis Riziotis, Antonina Andreeva, Delhi Kalwan, Jennifer De Jong, Philip Hinchliffe, Fabio Parmeggiani, Paul R. Race, Steven G. Burston, Alex Bateman, Rob Barringer, "A global survey of intramolecular isopeptide bonds", Protein Science, 34 (2025), DOI: 10.1002/pro.70342
- Julie E. M. Mcgeoch, Malcolm W. Mcgeoch, "Space-filling efficiency and optical properties of hemoglycin", Monthly Notices Of The Royal Astronomical Society, 543, 2338 - 2348 (2025), DOI: 10.1093/mnras/staf1484
- Jakub Luptak, Dean Clift, Aamir Mukadam, Jonathan Benn, Tyler Rhinesmith, Stephen H. Mclaughlin, Amy C. Dodds, Jerson E. Lapetaje, Matylda Sczaniecka-Clift, David J. France, William A. Mcewan, Leo C. James, "State-selective small molecule degraders that preferentially remove aggregates and oligomers", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-65454-z
- Tosca Holtrop, Arianne M. Brandsma, Louris J. Feitsma, Steffen Krohn, Petra Moerer, Frederique Van Den Haak, Anouk Versnel, Leonie Voss, Elsemieke M. Passchier, Maaike Nederend, J. H. Marco Jansen, Anouk G. Van Mourik, Rolf T. Urbanus, Diane Van Der Woude, Roger E. G. Schutgens, Rene E. M. Toes, Bert J. C. Janssen, Anja Lux, Kevin Budding, Matthias Peipp, Jeanette H. W. Leusen, "Preclinical assessment of two FcγRI-specific antibodies that competitively inhibit immune complex-FcγRI binding to suppress autoimmune responses", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-65133-z
- [Thesis] Jack R. Stubbs, "Droplet microfluidic and microcrystal strategies for time-resolved serial crystallography of enzymes at synchrotron and XFEL sources" (2025)
- Natalia Venetz-Arenas, Tim Schulte, Sandra Müller, Karin Wallden, Stefanie Fischer, Tom Resink, Nadir Kadri, Maria Paladino, Nicole Pina, Filip Radom, Denis Villemagne, Sandra Bruckmaier, Andreas Cornelius, Tanja Hospodarsch, Evren Alici, Hans-Gustaf Ljunggren, Benedict J. Chambers, Xiao Han, Renhua Sun, Marta Carroni, Victor Levitsky, Tatyana Sandalova, Marcel Walser, Adnane Achour, "Development of DARPin T cell engagers for specific targeting of tumor-associated HLA/peptide complexes", Iscience (2025), DOI: 10.1016/j.isci.2025.113926
- Hui Cao, Thomas R. Ward, Kailin Zhang, Vladimir Gorbachev, Tobias Vornholt, Kun Yu, Dongping Chen, "An asymmetric hydrogen atom transferase with an abiological thiophenol cofactor", Journal Of The American Chemical Society (2025), DOI: 10.1021/jacs.5c12516
- [Thesis] Benjamin W. Nash, "The structure and function of extracellular redox enzymes" (2025)
- Mingda Ye, Mpho Makola, Mark W. Richards, Joseph A. Newman, Michael Fairhead, Selena G. Burgess, Zhihuang Wu, Elizabeth Maclean, Nathan D. Wright, Lizbe Koekemoer, Andrew Thompson, Gustavo Arruda Bezerra, Gangshun Yi, Huanyu Li, Victor Rangel, Dimitrios Mamalis, Hazel Aitkenhead, Benjamin G. Davis, Robert J. C. Gilbert, Katharina L. Duerr, Richard Bayliss, Opher Gileadi, Frank Von Delft, "Gluebodies offer a route to improve crystal reliability and diversity through transferable nanobody mutations that introduce constitutive close contacts", Acs Central Science (2025), DOI: 10.1021/acscentsci.5c00937
- Alessio Ciulli, Peter Ettmayer, Kirsten Mcaulay, Vesna Vetma, Ilaria Puoti, Natalia Karolak, Sohini Chakraborti, Emelyne Diers, Enrico Girardi, Shakil Khan, Giorgia Kidd, Katrin G. Kropatsch, Ross Mclennan, Suzanne O’connor, Matthias Samwer, Nicole Trainor, Claire Whitworth, Andre J. Wijaya, Jeff Y. F. Wong, David Zollman, William Farnaby, Johannes Popow, "Identification of a highly cooperative PROTAC degrader targeting GTP-loaded KRAS(On) alleles", Journal Of The American Chemical Society (2025), DOI: 10.1021/jacs.5c10354
- Sophie J. L. Brown, David C. Briggs, Patrick Costello, Hiroko Yaguchi, Charles R. M. Bangham, Peter J. Parker, Neil Q. Mcdonald, "Penetrant PKCb mutation in ATLL displays a mixed gain-of-function", Biochemical Journal (2025), DOI: 10.1042/BCJ20253384
- [Conference Paper] Graeme Winter, Nick E. Devenish, James O'Hea, Gary Yendell, "Climbing mountains: building a data capture and correction system for JUNGFRAU 9M" (2025), DOI: 10.1063/4.0000827
- Sandra Codonya, Beatrice Jora, Miriam Santos-Caballero, Qiongju Qiu, Carla Calvó-Tusell, Celia Escriche, Andreea L. Turcu, Filippo Prischi, Clara Bartra, Cristina Val, Christophe Morisseau, Belén Pérez, Andrea Bertran-Mostazo, Sílvia Osuna, Rubén Corpas, Christian Griñán-Ferré, Carles Galdeano, M. Isabel Loza, Mercè Pallàs, Coral Sanfeliu, Bruce D. Hammock, José Brea, Ferran Feixas, Maria R. Conte, Enrique J. Cobos, Santiago Vázquez, "Dimethoxybenzohomoadamantane-based soluble epoxide hydrolase inhibitors: in vivo efficacy in a murine model of chemotherapy-induced neuropathic pain", Journal Of Enzyme Inhibition And Medicinal Chemistry (2025), DOI: 10.1080/14756366.2025.2574990
- Sean L. Beckwith, Matthew A. Cottee, J. Adam Hannon-Hatfield, Abigail C. Newman, Emma C. Walker, Justin R. Romero, Jonathan P. Stoye, Ian A. Taylor, David J. Garfinkel, "Probing the molecular determinants of Ty1 retrotransposon restriction specificity in yeast", Plos Genetics, 21 (2025), DOI: 10.1371/journal.pgen.1011898
- Paul A. Glossop, Paul Brear, Susanne Wright, Neil Flanagan, Melanie S. Glossop, Charlotte A. L. Lane, Richard P. Butt, David R. Spring, Marko Hyvonen, Darren Cawkill, "Exploiting the cryptic αd pocket of casein kinase 2α (CK2α) to deliver highly potent and selective Type 1 inhibitors", Journal Of Medicinal Chemistry (2025), DOI: 10.1021/acs.jmedchem.5c01807
- Cassandra Kennedy, Diego Esposito, Jessica Huber, David House, Katrin Rittinger, "Conformational dynamics of the bacterial E3 ligase SspH1", Journal Of Biological Chemistry, 169 (2025), DOI: 10.1016/j.jbc.2025.110671
- Ning Ma, Supriyo Bhattacharya, Sanychen Muk, Zuzana Jandova, Philipp S. Schmalhorst, Soumadwip Ghosh, Keith Le, Emelyne Diers, Nicole Trainor, William Farnaby, Michael J. Roy, Christiane Kofink, Peter Greb, Harald Weinstabl, Alessio Ciulli, Gerd Bader, Kyra Sankar, Andreas Bergner, Nagarajan Vaidehi, "Frustration in the protein-protein interface plays a central role in the cooperativity of PROTAC ternary complexes", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-63713-7
- Peter Smyth, Sofia Jaho, Lewis J. Williams, Gabriel Karras, Ann Fitzpatrick, Amy J. Thompson, Sinan Battah, Danny Axford, Sam Horrell, Marina Lucic, Kotone Ishihara, Machika Kataoka, Hiroaki Matsuura, Kanji Shimba, Kensuke Tono, Takehiko Tosha, Hiroshi Sugimoto, Shigeki Owada, Michael A. Hough, Jonathan A. R. Worrall, Robin L. Owen, "Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide", Iucrj, 12 (2025), DOI: 10.1107/S2052252525006645
- Conchita Fraguas Bringas, Mohd Syed Ahanger, Joyceline Cuenco, Hongling Liu, Alex B. Addinsall, Maria Lindahl, Ashley J. Ovens, Mark A. Febbraio, Marc Foretz, Olga Göransson, John W. Scott, Elton Zeqiraj, Kei Sakamoto, "Mechanism and cellular actions of the potent AMPK inhibitor BAY-3827", Science Advances, 11 (2025), DOI: 10.1126/sciadv.adx2434
- Mariah A. Cook, Jonathan D. Smailys, Ke Ji, Shelby M. Phelps, Jasmine N. Tutol, Wantae Kim, Whitney S. Y. Ong, Weicheng Peng, Caden Maydew, Y. Jessie Zhang, Sheel C. Dodani, "NitrOFF: An engineered fluorescent biosensor to illuminate nitrate transport in living cells", Angewandte Chemie International Edition, 135 (2025), DOI: 10.1002/anie.202508058
- S. Joe Russell, Catherine R. Back, Christopher Perry, Kaiman A. Cheung, Laurence Maschio, Sacha N. Charlton, Nicholas R. Lees, Monserrat Manzo-Ruiz, Martin A. Hayes, Marc W. Van Der Kamp, Paul R. Race, Christine L. Willis, "Chemoenzymatic total synthesis of the antibiotic (−)-13-deoxytetrodecamycin using the Diels–Alderase TedJ", Chemical Science, 48 (2025), DOI: 10.1039/D5SC05480J
- Hans E. Pfalzgraf, Aditya G. Rao, Kakali Sen, Hannah R. Adams, Marcus Edwards, You Lu, Chin Yong, Sofia Jaho, Takehiko Tosha, Hiroshi Sugimoto, Sam Horrell, James Beilsten-Edmands, Robin L. Owen, Colin R. Andrew, Jonathan A. R. Worrall, Ivo Tews, Adrian J. Mulholland, Michael A. Hough, Thomas W. Keal, "Double crossed? Structural and computational studies of an unusually crosslinked haem in Methylococcus capsulatus cytochrome P460", Chemical Science (2025), DOI: 10.1039/D5SC04213E
- Xiyue Leng, Katherine I. Albanese, Lia R. Golub, Arthur A. Norman, Jonathan Clayden, Derek Woolfson, "De novo designed 3-helix bundle peptides and proteins with controlled topology and stability", Chemical Science (2025), DOI: 10.1039/D5SC05576H
- Alejandro Morales-Florez, Colin W. J. Lockwood, Benjamin W. Nash, Marcus J. Edwards, Jessica H. Van Wonderen, Amit Sachdeva, Julea N. Butt, Thomas A. Clarke, "Extracellular catalysis of environmental substrates by Shewanella oneidensis MR ‐1 occurs via active sites on the C‐terminal domains of MtrC", Protein Science, 34 (2025), DOI: 10.1002/pro.70243
- Joseph F. Hoff, Kirsty E. Goudar, Karina Calvopina, Michael Beer, Philip Hinchliffe, John M. Shaw, Catherine L. Tooke, Yuiko Takebayashi, Andrew F. Cadzow, Nicholas Harmer, Adrian J. Mulholland, Christopher J. Schofield, James Spencer, "Electrostatic interactions influence diazabicyclooctane inhibitor potency against OXA-48-like β-lactamases", Rsc Medicinal Chemistry (2025), DOI: 10.1039/D5MD00512D
- Jack W. J. Welland, Henry G. Barrow, Phillip J. Stansfeld, Janet E. Deane, "Conformational dynamics and membrane insertion mechanism of B4GALNT1 in ganglioside synthesis", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-60593-9
- María Ángeles Márquez-Moñino, Ana Martínez Gascueña, Tala Azzam, Andrea Persson, Aitor Manzanares-Gomez, Marina Aguillo-Urarte, Trenton T. Brown, Ainhoa Montero-Sagarminaga, Rolf Lood, Andreas Naegeli, Sean R. Connell, Diego E. Sastre, Eric J. Sundberg, Beatriz Trastoy, "Molecular basis of Fab-dependent IgA antibody recognition by gut-bacterial metallopeptidases", The Embo Journal, 630 (2025), DOI: 10.1038/s44318-025-00518-w
- Morgan Thomas, Pierre G. Matricon, Robert J. Gillespie, Maja Napiórkowska, Hannah Neale, Jonathan S. Mason, Jason Brown, Kaan Harwood, Charlotte Fieldhouse, Nigel A. Swain, Tian Geng, Noel M. O’boyle, Francesca Deflorian, Andreas Bender, Chris De Graaf, "Identification of nanomolar adenosine A2A receptor ligands using reinforcement learning and structure-based drug design", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-60629-0
- Wantae Kim, Po-Hsun Fan, Junling Bu, Hung-Wen Liu, Y. Jessie Zhang, "Structure-guided mechanistic investigation of stereochemical inversion during the octose formation catalyzed by an atypical transaldolase AprG in the biosynthesis of apramycin", Acs Catalysis (2025), DOI: 10.1021/acscatal.5c03420
- Ramon Hurtado-Guerrero, Spyridon Gatos, Irene Ginés-Alcober, Javier Macías-León, Andrés Manuel González-Ramírez, Ilknur Kasapoglu, Billy Veloz, Ismael Compañón, Mattia Ghirardello, Pedro Merino, Francisco Corzana, Ola Blixt, "Structure-guided phage display discovery of antibodies for (S)Tn-glycans in protein context", Nature Chemical Biology, 13 (2025), DOI: 10.1038/s41589-025-01971-8
- Emmanuel Matabaro, Haigang Song, Lukas Sonderegger, Fabio Gherlone, Andrew M. Giltrap, Sam Liver, Alvar D. Gossert, Markus Künzler, James H. Naismith, "Macrocyclization of backbone N-methylated peptides by a prolyl oligopeptidase with a distinctive substrate recognition mechanism", Chemical Science, 57 (2025), DOI: 10.1039/D5SC03723A
- Madeline E. Kavanagh, Kirsty J. Mclean, Sophie H. Gilbert, Cecilia N. Amadi, Matthew Snee, Richard B. Tunnicliffe, Kriti Arora, Helena I. M. Boshoff, Alexander Fanourakis, Maria Jose Rebollo-Lopez, Fatima Ortega, Colin W. Levy, Andrew W. Munro, David Leys, Chris Abell, Anthony G. Coyne, "Fragment-based development of small molecule inhibitors targeting Mycobacterium tuberculosis cholesterol metabolism", Journal Of Medicinal Chemistry (2025), DOI: 10.1021/acs.jmedchem.5c00478
- Tomas Akerud, Claudia De Fusco, Peter Brandt, Fredrik Bergström, Patrik Johansson, Margareta Ek, Ulf Börjesson, Anders Johansson, Jakob Danielsson, Martin Bauer, Bertrand Arnaud, Marie Castaldo, Maria Strömstedt, Birgitta Rosengren, Frank Jansen, Linda Fredlund, "Mechanism and kinetics of turnover inhibitors of nicotinamide N-methyl transferase in vitro and in vivo", Journal Of Biological Chemistry, 301 (2025), DOI: 10.1016/j.jbc.2025.108492
- Guido C. Paesen, Weng M. Ng, Simon Kimuda, Geoff Sutton, Katie J. Doores, Thomas A. Bowden, "Structure and stabilization of the antigenic glycoprotein building blocks of the New World mammarenavirus spike complex", Mbio (2025), DOI: 10.1128/mbio.01076-25
- Zak Mciver, Alicia Moraleda-Montoya, Zongjia Chen, Ruwan Epa, David Starns, Matthew Davy, Mikel Garcia-Alija, Arnaud Basle, Mario Schubert, Didier Ndeh, Beatriz Trastoy, Spencer J. Williams, Marcelo E. Guerin, Alan Cartmell, "Understanding the substrate recognition and catalytic mechanism of methyl fucosidases from glycoside hydrolase family 139", Journal Of Biological Chemistry, 11 (2025), DOI: 10.1016/j.jbc.2025.110407
- Amy J. Thompson, James Beilsten-Edmands, Cicely Tam, Juan Sanchez-Weatherby, James Sandy, Halina Mikolajek, Danny Axford, Sofia Jaho, Michael A. Hough, Graeme Winter, "Enhanced intensity-based clustering of isomorphous multi-crystal data sets in the presence of subtle variations", Acta Crystallographica Section D Structural Biology, 81, 278 - 290 (2025), DOI: 10.1107/S2059798325004589
- Geoffrey Masuyer, Andreas Rummel, Pal Stenmark, "Botulinum neurotoxin A mutants with enhanced ganglioside binding show improved potency and altered ganglioside selectivity", Communications Chemistry, 8 (2025), DOI: 10.1038/s42004-025-01569-0
- [Conference Paper] Yelyzaveta Pulnova, Briony Alissa Yorke, "HATRX for radiation damage study" (2025)
- Wenjie Chen, "Fragment-based drug discovery for transthyretin kinetic stabilisers using a novel capillary zone electrophoresis method", Plos One, 20 (2025), DOI: 10.1371/journal.pone.0323816
- Zehui Duan, Jiaao Wei, Stephen B. Carr, Miguel Ramirez, Rhiannon M. Evans, Philip A. Ash, Patricia Rodriguez-Macia, Amit Sachdeva, Kylie A. Vincent, "Cyanophenylalanine as an infrared probe for iron-sulfur cluster redox state in multicenter metalloenzymes", Chembiochem, 2 (2025), DOI: 10.1002/cbic.202500251
- [Thesis] Samuel P. Foster, "A combined methodology for radiation damage mitigation and enzyme intermediate study in macromolecular crystals" (2025), DOI: 10.25392/leicester.data.29155316.v1
- Sieglinde De Cae, Inge Van Molle, Loes Van Schie, Sophie R. Shoemaker, Julie Deckers, Nincy Debeuf, Sahine Lameire, Wim Nerinckx, Kenny Roose, Daria Fijalkowska, Simon Devos, Anne-Sophie De Smet, Jackeline Cecilia Zavala Marchan, Toon Venneman, Koen Sedeyn, Lejla Mujanovic, Marlies Ballegeer, Manon Vanheerswynghels, Caroline De Wolf, Hans Demol, Jasper Zuallaert, Pieter Vanhaverbeke, Gholamreza Hassanzadeh Ghassabeh, Chiara Lonigro, Viki Bockstal, Manuela Rinaldi, Rana Abdelnabi, Johan Neyts, Susan Marqusee, Bart N. Lambrecht, Nico Callewaert, Han Remaut, Xavier Saelens, Bert Schepens, "Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-60250-1
- Gyles E. Cozier, Lauren B. Coulson, Charles J. Eyermann, Gregory S. Basarab, Sylva L. Schwager, Kelly Chibale, Edward D. Sturrock, K. Ravi Acharya, "Design of novel mercapto-3-phenylpropanoyl dipeptides as dual angiotensin-converting enzyme C–domain-selective/neprilysin inhibitors", Journal Of Medicinal Chemistry (2025), DOI: 10.1021/acs.jmedchem.5c00329
- William S. Stuart, Christopher H. Jenkins, Philip M. Ireland, Michail N. Isupov, Isobel H. Norville, Nicholas J. Harmer, "Structure and catalytic mechanism of methylisocitrate lyase, a potential drug target against Coxiella burnetii", Journal Of Biological Chemistry, 42 (2025), DOI: 10.1016/j.jbc.2025.108517
- Chiara Bosetti, Dionysis Kampasis, Shoshy A. Brinch, Albert Galera-Prat, Maria Karelou, Saurabh S. Dhakar, Juho Alaviuhkola, Jo Waaler, Lari Lehtio, Ioannis K. Kostakis, "Substitutions at the C-8 position of quinazolin-4-ones improve the potency of nicotinamide site binding tankyrase inhibitors", European Journal Of Medicinal Chemistry, 288 (2025), DOI: 10.1016/j.ejmech.2025.117397
- Owens Uwangue, Johan Glerup, Andreas Dunge, Monika Bjelcic, Gabrielle Wehlander, Gisela Branden, "Microcrystallization and room-temperature serial crystallography structure of human cytochrome P450 3A4", Archives Of Biochemistry And Biophysics, 28 (2025), DOI: 10.1016/j.abb.2025.110419
- Camilla Gottlieb Andersen, Laust Bavnhøj, Søren Brag, Anastasiia Bohush, Adriana Chrenkova, Jan Heiner Driller, Bjorn Panyella Pedersen, "Comparative analysis of STP6 and STP10 unravels molecular selectivity in sugar transport proteins", Proceedings Of The National Academy Of Sciences, 122 (2025), DOI: 10.1073/pnas.2417370122
- Pramila Rijal, Leiyan Wei, Guido C. Paesen, David I. Stuart, Mark R. Howarth, Kuan-Ying A Huang, Thomas A. Bowden, Alain R. M. Townsend, "Structure-guided loop grafting improves expression and stability of influenza neuraminidase for vaccine development", Elife (2025), DOI: 10.7554/eLife.105317
- Joel J. Chubb, Katherine I. Albanese, Alison Rodger, Derek N. Woolfson, "De novo design of parallel and antiparallel A3B3heterohexameric α-helical barrels", Biochemistry (2025), DOI: 10.1021/acs.biochem.4c00584
- Fouzia Bano, Suneale Banerji, Tao Ni, Dixy E. Green, Kalila R. Cook, Iain W. Manfield, Paul L. Deangelis, Emanuele Paci, Martin Lepšík, Robert J. C. Gilbert, Ralf P. Richter, David G. Jackson, "Structure and unusual binding mechanism of the hyaluronan receptor LYVE-1 mediating leucocyte entry to lymphatics", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-57866-8
- Chrislaine Withers-Martinez, Roger George, Roksana Ogrodowicz, Simone Kunzelmann, Andrew G. Purkiss, Svend Kjaer, Philip A. Walker, Vadims Kovada, Aigars Jirgensons, Michael J. Blackman, "Structural plasticity of Plasmodium falciparum plasmepsin X to accommodate binding of potent macrocyclic hydroxyethylamine inhibitors", Journal Of Molecular Biology, 16 (2025), DOI: 10.1016/j.jmb.2025.169062
- Virginie Will, Lucile Moynie, Elise Si Ahmed Charrier, Audrey Le Bas, Lauriane Kuhn, Florian Volck, Johana Chicher, Hava Aksoy, Morgan Madec, Cyril Antheaume, Gaëtan L. A. Mislin, Isabelle J. Schalk, "Structure of the outer membrane transporter fema and its role in the uptake of ferric dihydro-aeruginoic acid and ferric aeruginoic acid in Pseudomonas aeruginosa", Acs Chemical Biology (2025), DOI: 10.1021/acschembio.4c00820
- Kiefer O. Ramberg, Coilin Boland, Hamed Kooshapur, Olivier Soubias, Maciej Wiktor, Chia-Ying Huang, Jonathan Bailey, Klaus Gawrisch, Martin Caffrey, "Solid-state NMR of membrane peptides and proteins in the lipid cubic phase", Biophysical Journal (2025), DOI: 10.1016/j.bpj.2025.03.012
- Liuyi Hu, Alan Wainman, Antonina Andreeva, Muladili Apizi, Ines Alvarez-Rodrigo, Siu-Shing Wong, Saroj Saurya, Devon Sheppard, Matthew Cottee, Steven Johnson, Susan M. Lea, Jordan W. Raff, Mark Van Breugel, Zhe Feng, "The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly", Science Advances, 11 (2025), DOI: 10.1126/sciadv.adr5744
- Linda Makhlouf, Mukul Mishra, Hannah Makhlouf, Iain Manfield, Luca Busino, Elton Zeqiraj, "Sequence rules for a long SPOP-binding degron required for protein ubiquitylation", Biochemical Journal (2025), DOI: 10.1042/BCJ20253041
- Mst Luthfun Nesa, Suman K. Mandal, Christine Toelzer, Diana Humer, Peter C. E. Moody, Imre Berger, Oliver Spadiut, Emma L. Raven, "Crystal structure of ferric recombinant horseradish peroxidase", Jbic Journal Of Biological Inorganic Chemistry, 2 (2025), DOI: 10.1007/s00775-025-02103-2
- Charles W. Parry, Francesca Pellicano, Alexander W. Schuettelkopf, Kim S. Beyer, Justin Bower, Amy Bryson, Kenneth Cameron, Nichole M. Cerutti, Jonathan P. Clark, Stuart C. Davidson, Keneth Davies, Martin J. Drysdale, Jeffrey Engelman, Anna Estevan-Barber, Andrea Gohlke, Christopher H. Gray, Daniel A. Guthy, Min Hong, Alana Hopkins, Luke D. Hutchinson, Jennifer Konczal, Michel Maira, Duncan Mcarthur, Mokdad Mezna, Heather Mckinnon, Ridvan Nepravishta, Nils Ostermann, Camila C. Pasquali, Katie Pollock, Angelo Pugliese, Nicholas Rooney, Niko Schmiedeberg, Paul Shaw, Camilo Velez-Vega, Christopher West, Ryan West, Frederic Zecri, John B. Taylor, "Reversible small molecule multivariant ras inhibitors display tunable affinity for the active and inactive forms of Ras", Journal Of Medicinal Chemistry, 49 (2025), DOI: 10.1021/acs.jmedchem.4c02929
- Mélanie Loiodice, Elodie Drula, Zak Mciver, Svetlana Antonyuk, Arnaud Basle, Marcelo Lima, Edwin A. Yates, Dominic P. Byrne, Jamie Coughlan, Andrew Leech, Shahram Mesdaghi, Daniel J. Rigden, Sophie Drouillard, William Helbert, Bernard Henrissat, Nicolas Terrapon, Gareth S. A. Wright, Marie Couturier, Alan Cartmell, "Bacterial polysaccharide lyase family 33: Specificity from an evolutionarily conserved binding tunnel", Proceedings Of The National Academy Of Sciences, 122 (2025), DOI: 10.1073/pnas.2421623122
- Andreas Luttens, Duc Duy Vo, Emma R. Scaletti, Elisée Wiita, Ingrid Almlöf, Olov Wallner, Jonathan Davies, Sara Kosenina, Liuzhen Meng, Maeve Long, Oliver Mortusewicz, Geoffrey Masuyer, Flavio Ballante, Maurice Michel, Evert Homan, Martin Scobie, Christina Kalderén, Ulrika Warpman Berglund, Andrii V. Tarnovskiy, Dmytro S. Radchenko, Yurii S. Moroz, Jan Kihlberg, Pål Stenmark, Thomas Helleday, Jens Carlsson, "Virtual fragment screening for DNA repair inhibitors in vast chemical space", Nature Communications, 16 (2025), DOI: 10.1038/s41467-025-56893-9
- [Thesis] Monse Manzo Ruiz, "Structural enzymology of spirotetronate cyclases" (2025)
- Shangwen Luo, Xin-Rong Li, Xiao-Tong Gong, Alexey Kulikovsky, Feng Qu, Konstantinos Beis, Konstantin Severinov, Svetlana Dubiley, Xinxin Feng, Shi-Hui Dong, Satish K. Nair, "Trojan horse peptide conjugates remodel the activity spectrum of clinical antibiotics", Proceedings Of The National Academy Of Sciences, 122 (2025), DOI: 10.1073/pnas.2319483121
- Audrey Le Bas, Bradley R. Clarke, Tanisha Teelucksingh, Micah Lee, Kamel El Omari, Andrew M. Giltrap, Stephen A. Mcmahon, Hui Liu, John H. Beale, Vitaliy Mykhaylyk, Ramona Duman, Neil G. Paterson, Philip N. Ward, Peter J. Harrison, Miriam Weckener, Els Pardon, Jan Steyaert, Huanting Liu, Andrew Quigley, Benjamin G. Davis, Armin Wagner, Chris Whitfield, James H. Naismith, "Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide", Open Biology, 15 (2025), DOI: 10.1098/rsob.240310
- [Thesis] Lea K. Drogalis, "Functional characterization of the polymerase domain of polymerase θ in humans and T. cruzi" (2025)
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