VMXi is open for user experiments. Please contact the beamline staff if you would like to collect data at VMXi. You may also select VMXi as part of your BAG application.
The Versatile Macromolecular Xtallography in-situ (VMXi) beamline is dedicated to room temperature data collection from macromolecular crystals. It is an entirely automated facility for characterisation of, and data collection directly from, crystallisation experiments in situ.
There are several key advantages of in situ diffraction:
Currently VMXi offers:
Which can help you with the following experiment types:
There are three modes of access to VMXi, all by prior arrangement with the VMXi team:
If you would like to collect room temperature data at VMXi, please contact the beamline team letting us know the problem you are trying to solve, how samples will be provided and by when so we can review what we can support.
If you require further details, do not hesitate to get in touch - VMXi@diamond.ac.uk
J. Sanchez-Weatherby, J. Sandy, H. Mikolajek, CMC. Lobley, M. Mazzorana, J. Kelly, G. Preece, R. Littlewood, TLM. Sørensen VMXi: a fully automated, fully remote, high-flux in situ macromolecular crystallography beamline Synchrotron Radiat. J. 26 (2019) 291-301.
H. Mikolajek, J. Sanchez-Weatherby, J. Sandy, R.J. Gildea, I. Campeotto, H. Cheruvara, J.D. Clarke, T. Foster, S. Fujii, I.T. Paulsen, B.S. Shahk & M.A. Hough Protein-to-structure pipeline for ambient-temperature in situ crystallography at VMXi ICUr 10 (2023) 420-429
|Techniques||Automated screening and interactive data collection of samples in standard Greiner Crystal QuickX ™ or MiTeGen In-Situ-1 ™ plates using wide band pass beam from multilayer-based monochromator.|
|Key performance parameters||
Ability to screen 500 1000 µm2 regions of interest in 24 hours. Collection of 4000 oscillation datasets in 24 hours. Accuracy between off-line and on-line position of samples should be better than 0.5 µm.
|Sample environment||20 °C sample environment. Local storage, sample visualisation,dedicated goniometer.|
|Robot/Sample manipulation||Plate handling robot for automated handling. 10 second exchange time between sample position and local storage. 1 minute exchange time between local storage and long-term storage.|
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