Biological Cryo-Imaging - Diamond Light Source

Welcome to Biological Cryo-Imaging

Biological imaging at Diamond now has a new home with the recent formation of the Biological Cryo-Imaging Group. This brings together dedicated facilities for X-ray, light and electron microscopy at Diamond. The bending magnet beamline B24 is the source of X-rays for the full field cryo-transmission X-ray microscope dedicated to biological X-ray imaging and the beamline has also established a cryo super resolution fluorescence microscopy facility which is a joint venture between Diamond and the University of Oxford. The Biological Cryo-Imaging Group is completed with the recently established national centre for cryo-electron microscopy at Diamond, eBIC (Electron Bio-Imaging Centre).

Instruments

Instruments -

B24 Cryo-TXM

Operational in optimisation mode

B24 is the full-field transmission microscope at Diamond designed specifically to meet the rising demand for tomographic imaging of biological specimens under near physiological conditions.

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Energy: 500 eV

Tomography Imaging Microscopy Cryo X-ray Microscopy CLEM: Correlative Light Electron Microscopy CLXM: Correlative Light X-ray Microscopy

eBIC

eBIC provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for both single particle analysis and cryo-tomography. Currently eBIC houses four Titan Krios microscopes, a Talos Arctica and a Scios cryo-FIB/SEM. The Polara microscope is located at the University of Oxford.

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Accelerating Voltages: 3-300 kV

Cryo-EM: Cryo Electron Microscopy Cryo-ET: Cryo Electron Tomography Humidity Control In situ Imaging Electron Microscope Electron Crystallography FIBSEM: Cryo Focused Ion Beam Scanning Electron Microscopy Microscopy