Macromolecular Crystallography (MX)
MX is a core activity at Diamond with seven beamlines dedicated to the technique alongside the XFEL Hub, Membrane Protein Laboratory and XChem fragment screening facility for the extensive UK structural biology community as well as researchers in Europe and beyond. The staff of the MX group are recognised as innovative world leaders in MX, moving the goalposts of what is feasible for 'conventional' MX as well as developing techniques and beamlines that transform MX to the next level, enabling new experiments and methodologies. The group takes a long term approach to enabling new capabilities at its suite of beamlines to meet the current and future demands of an exacting community of scientists.
I02-1 VMXmUnder construction
VMXm is a micro/nanofocus MX beamline aimed at atomic structure determination in cases where the production of significant quantities of protein material and crystals is problematic.More information
Wavelength: 0.57 - 2.0 Å
Energy: 7.0 - 25.0 keV
The Versatile Macromolecular X-tallography in-situ (VMXi) beamline will be an entirely automated facility for characterisation of, and data collection directly from, crystallisation experiments in situ.More information
Energy: 12 keV
High throughput and highly automated beamline for optimised MAD and SAD experiments. Capable of accepting CL3 type experiments on crystals of pathogens.More information
Detector: Eiger2 XE 16M
Wavelength: 0.5 - 2.5 Å
I04 Microfocus MX
Variable focus from 5 to 100 microns, high throughput and highly automated beamline for optimised MAD and SAD experiments.More information
Detector: Eiger2 XE 16M
Wavelength: 0.69 - 2.07 Å
I04-1 Fixed wavelength MX
High throughput and highly automated fixed wavelength SAD beamline for macromolecular crystallography.More information
Detector: Pilatus 6M-F
Wavelength: 0.92Å (fixed)
I23 Long-Wavelength MXCommissioning
A unique facility for solving the crystallographic phase problem, using the small anomalous signals from sulphur or phosphorous which are present in native protein or RNA/DNA crystals. Additionally, anomalous difference Fourier maps can be used to locate sulphur and phosphorous positions to assist model building at low resolution and/or identify lighter atoms such as chlorine, potassium and calcium.More information
Energy: 2.1 – 11 keV
I24 Microfocus MX
High throughput variable microfocus beamline for optimised MAD and SAD experiments on crystals down a few microns in size.More information
Detector: Pilatus3 6M
Wavelength: 0.62Å - 1.77Å
Membrane Protein Laboratory
The Membrane Protein Lab (MPL) at Diamond is a research and training facility for scientists interested in solving the 3D structures of membrane proteins by X-ray crystallography.More information
The UK is taking a leading role in the development of a new structural biology facility (SFX) at the European X-ray Free Electron Laser (XFEL), in Hamburg, Germany, and a complementary facility at Diamond (The UK XFEL Hub) to help develop the required expertise.More information
|Wavelength range (Å)||0.6 - 2.3||0.69 - 2.07||0.92 (fixed)||0.7 - 2.0|
|Energy range (keV)||5.2 - 21.0||6.0 - 18.0||13.5 (fixed)||6.4 - 20.0|
|Default settings (Å/keV)||0.98 / 12.7||0.98 / 12.66||0.92 / 13.53||0.97 / 12.8|
|Flux (ph/s) in full beam at default energy at 300 mA||1.7 x 1012||3.0 x 1011||9.0 x 1011||3.0 x 1012|
|Default beamsize (µm)||90 x 20||30 x 20||60 x 50||8 x 8|
|Full beam size at sample (µm)||90 x 20, 90 x 30, 100 x 100, 55 x 10||10 x 5 --> 110 x 100||60 x 50||
5 x 5 --> 50 x 40
|Available apertures (µm)||
20, 50, 100
|10, 20, 30, 50 and 70|
|Detectors (Dectris type)||
Eiger2 XE 16M
Eiger2 XE 16M
|Number of unipucks / SPINE pins||37/592||37 / 592||37 / 592||37 / 592|
|Maximum samples / hour||30||30||30||30|
|Typical samples / hour||15 - 25||15 - 25||15 - 35||15 - 25|
|Minimum Detector Distance (mm)||140||170||159||250||200|
Maximum Resolution For Each Beamline
Reserve access well ahead of your visit either at the links in the web page or via your local contact to facilities such as
Check the Diamond MX web pages and individual beamline pages for updates, manuals and contact details prior to your experiment.
Register your shipping dewars with Diamond to use our shipping account and help with tracking.
Always purchase and use SPINE pins and Unipucks to mount and ship your samples. Non-SPINE standard pins cause robots to crash and damage to equipment: you lose beamtime, we lose sleep.
For full remote access experiments we prefer pucks to arrive in the shelved shipping cane or alternatively the puck shipping canister. Please avoid the narrow canisters where possible.
Understand the visit timings you are scheduled for.
For remote experimenters read the remote access guidelines.
- For remote access experiments the team leader should update UAS with contact details for all remote users, and ensure all pucks are correctly assigned on ISPyB at least two working days before the visit.
- For mixed remote access (i.e. where a member of your team is on-site) ensure they will be available to change pucks if required.
- In both cases discuss your requirements for remote access with your local contact before your experiment.
For all experiments we highly recommend preparing your shipments in advance in ISPyB, identifying your pucks by the puck identifier (e.g. CPS-0024) and assigning samples at the same time. This allows pucks to be assigned to robot positions as loaded by either you or Diamond staff making them available in GDA immediately. For remote access this is a requirement rather than a recommendation to ensure reliable information transfer.
For UK users with Diamond registered dewars you can book your next working day shipping to us with Diamond’s DHL account. We recommend shipping your dewars three or more days before your experiment to ensure arrival in time.
Register your PDB and sequences for your proteins in ISPyB. These can then be used automatically if you use default file names in GDA for all future visits in downstream processing such as XIA2, DIMPLE and MrBUMP and avoids the need to do this for each visit.
Refer to the manual for data collection possibilities –
- grid scans - find your crystals or the best diffracting region of a crystal and centre reliably
- line scans – minimise radiation damage, maximise resolution
- fluorescence data – find out if you have metals and what energy to use to exploit for phasing
- in-situ experiments - test crystal and collect data in trays
- dehumidifier HC1 - optimise diffraction by adjusting humidity
- data collections – optimise your data collections:
- multi axis goniometry
- inverse beam
- interleaved MAD
- washing and annealing crystals and other tools.
Read the message of the day in GDA for beamline status.
Refer to beamline specific manuals where appropriate.
Start your data back-up as soon as you can.
Load your pre-registered pucks and assign positions on the touch screen in the hutch (soon in all beamlines). Now start GDA and all your samples will be preloaded in the sample changer view (or press refresh to update if GDA was already running).
Use default file names in GDA to streamline experiments, avoiding the need to type information at each sample load, preventing mistakes and ensuring downstream processing uses the information you have provided in advance (PDB, sequence, space group).
Talk with your local contact about your experiment plans – we are here to help you solve structures.
Specialised high flux microfocus beams for microcrystal data collection or the illumination of subvolumes of larger inhomogeneous crystals.More information...
The highly automated beamlines enable data collection from anywhere in the world via remote access. Systems are in place for shipping samples to and from Diamond Light Source. New users can borrow pucks and tools.More information...
In situ Data Collection
In situ data collection and sample characterization directly from crystals in their crystallization plates.More information...
Sample Humidity Control
Control of the sample crystal's relative humidity with the aim of improving diffraction quality through improved packing of protein molecules constituting the lattice.More information...
On-line and off-line UV-Vis spectroscopy of macromolecular samples to study enzyme reaction mechanisms and radiation damage.More information...
Crystal reorientation to optimize data collection strategies for anomalous diffraction data collection and low symmetry spacegroups, or very long axis unit cells.More information...
All beamlines are designated bio containment level 1. For containment level 2 samples beamlines I03 and I24 can be used and beamline I03 can be configured to run at biocontainment level 3.More information...