Dehumidifier HC1

The HC1b is a device developed by the Instrumentation Group at the EMBL outstation at Grenoble. It has been thoroughly tested at the ESRF at ID14-2 and BM14. The device is now at Diamond and can be setup on demand at some of the MX beamlines. In addition HC1 can be used offline in a dedicated lab prior to your beamtime. 

Please contact [email protected] if you would like more information on technical aspects of the experiments and to request its use.

References (HC1)

Sanchez-Weatherby, J., M. W. Bowler, et al. (2009). "Improving diffraction by humidity control: a novel device compatible with X-ray beamlines." Acta Crystallographica Section D 65(12): 1237-1246.

Russi, S., D. H. Juers, et al. (2011). "Inducing phase changes in crystals of macromolecules: status and perspectives for controlled crystal dehydration." J Struct Biol 175(2): 236-243.

Pellegrini, E., D. Piano, et al. (2011). "Direct cryocooling of naked crystals: are cryoprotection agents always necessary?" Acta Crystallogr D Biol Crystallogr 67(10): 902-906.

Hu, N. J., S. Iwata, et al. (2011). "Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT." Nature 478(7369): 408-411.

Kadlec, J., E. Hallacli, et al. (2011). "Structural basis for MOF and MSL3 recruitment into the dosage compensation complex by MSL1." Nat Struct Mol Biol 18(2): 142-149.

Abad-Zapatero, C., R. Oliete, et al. (2011). "Humidity control can compensate for the damage induced in protein crystals by alien solutions." Acta Crystallographica Section F 67(10): 1300-1308.

Pellegrini, E., D. Piano, et al. (2011). "Direct cryocooling of naked crystals: are cryoprotection agents always necessary?" Acta Crystallogr D Biol Crystallogr 67(10): 902-906.

Zerrad, L., A. Merli, et al. (2011). "A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase." J Biol Chem 286(16): 14040-14048.

Wheeler, M. J., S. Russi, et al. (2012). "Measurement of the equilibrium relative humidity for common precipitant concentrations: facilitating controlled dehydration experiments." Acta Crystallogr Sect F Struct Biol Cryst Commun 68(1): 111-114.

Mueller, U., N. Darowski, et al. (2012). "Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin." J Synchrotron Radiat 19(3): 442-449.
 

References (Dehydration)

Russo Krauss, I., F. Sica, et al. (2012). "Increasing the X-ray Diffraction Power of Protein Crystals by Dehydration: The Case of Bovine Serum Albumin and a Survey of Literature Data." Int J Mol Sci 13(3): 3782-3800.

Deng, X., W. S. Davidson, et al. (2012). "Improving the diffraction of apoA-IV crystals through extreme dehydration." Acta Crystallogr Sect F Struct Biol Cryst Commun 68(1): 105-110.

Newman, J. (2006). "A review of techniques for maximizing diffraction from a protein crystal in stilla." Acta Crystallogr D Biol Crystallogr 62(1): 27-31.

Bowler, M. W., M. G. Montgomery, et al. (2006). "Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F(1)-ATPase by controlled dehydration." Acta Crystallogr D Biol Crystallogr 62(9): 991-995.

Heras, B. and J. L. Martin (2005). "Post-crystallization treatments for improving diffraction quality of protein crystals." Acta Crystallogr D Biol Crystallogr 61(9): 1173-1180.

Kalinin, Y., J. Kmetko, et al. (2005). "A new sample mounting technique for room-temperature macromolecular crystallography." Journal of Applied Crystallography 38(2): 333-339.

Kalinin, Y. and R. Thorne (2005). "Crystal growth in X-ray-transparent plastic tubing: an alternative for high-throughput applications." Acta Crystallogr D Biol Crystallogr 61(11): 1528-1532.

Heras, B., M. A. Edeling, et al. (2003). "Dehydration converts DsbG crystal diffraction from low to high resolution." Structure 11(2): 139-145.

Kiefersauer, R., M. E. Than, et al. (2000). "A novel free-mounting system for protein crystals: transformation and improvement of diffraction power by accurately controlled humidity changes." Journal of Applied Crystallography 33(5): 1223-1230.

Kiefersauer, R., J. Stetefeld, et al. (1996). "Protein-Crystal Density by Volume Measurement and Amino-Acid Analysis." Journal of Applied Crystallography 29(4): 311-317.