Quick Guide to Using I23
Check the Diamond I23 web pages for updates, manuals and contact details prior to your experiment.
Before applying for I23 beamtime, users are asked to fill in a questionnaire related to their project in order to allow beamline staff to assess the feasibility of the experiment. Once the questionnaire has been reviewed, users can request from the beamline sample holders of different sizes and combi vial pucks.
Register your shipping dewars with Diamond to use our shipping account and help with tracking.
Always use I23 custom made pins and vial pucks to mount and ship your samples.
Understand the visit timings you are scheduled for.
For experimenters coming on-site simplify your arrival by taking the Fast Track approach.
For all experiments we highly recommend preparing your shipments in advance in ISPyB, identifying your pucks by the puck identifier (e.g. CPS-0024) and assigning samples at the same time. This allows pucks to be assigned to robot positions as loaded by either you or Diamond staff making them available in GDA immediately.
For UK users with Diamond registered dewars you can book your next working day shipping to us with Diamond’s DHL account. We recommend shipping your dewars three or more days before your experiment to ensure arrival in time.
Register your PDB and sequences for your proteins in ISPyB. These can then be used automatically if you use default file names in GDA for all future visits in downstream processing such as XIA2, DIMPLE and MrBUMP and avoids the need to do this for each visit.
Refer to the manual for data collection possibilities –
- fluorescence data – find out if you have metals and what energy to use to exploit for phasing
- data collections – optimise your data collections:
- Multi axis gonimeter
- inverse beam
- interleaved MAD
Read the message of the day in GDA for beamline status.
Refer to beamline specific manuals where appropriate.
Start your data back-up as soon as you can.
Load your pre-registered pucks and assign positions on the touch screen in the hutch (soon in all beamlines). Now start GDA and all your samples will be preloaded in the sample changer view (or press refresh to update if GDA was already running).
Use default file names in GDA to streamline experiments, avoiding the need to type information at each sample load, preventing mistakes and ensuring downstream processing uses the information you have provided in advance (PDB, sequence, space group).
Talk with your local contact about your experiment plans – we are here to help you solve structures.
Unload your last sample with the sample changer with sufficient time to ensure you have removed pucks from the sample changers before the end of your shift (unless you have discussed a time extension with the local contact).
Finalise your backup disk and unmount cleanly in the data dispenser interface.
Complete your dewar dispatch form for storage at Diamond or return of your dewars. You can use our shipping agents for dewar return.
Diamond pays for sample shipping (2 dewars per shift) but for UK users only if you use our shipping agents via our webpages. No reimbursement of UK shipping will be made after August 2014 for shipping you arrange yourself.
You can reprocess your data afterwards on our cluster.