Macromolecular Crystallography (MX)
The Diamond MX village currently comprises four state-of-the-art undulator beamlines - two high brilliance MAD beamlines (I03, I04), a fixed wavelength beamline (I04-1) and a microfocus MAD beamline (I24). We offer a wide range of facilities and capabilities including microbeams, in situ (in-plate) screening and data collection, sample humidity control, UV-Vis spectroscopy and data collection from pathogenic samples.
- remote access data collection
- fast sample changers accepting SPINE standard pins in Unipucks
- fully automated data analysis (Xia2, DIMPLE, FastDP, FastEP, MrBUMP)
- state-of-the-art Pilatus Pixel Array Detectors
Specialised high flux microfocus beams for microcrystal data collection or the illumination of subvolumes of larger inhomogeneous crystals.
The highly automated beamlines enable data collection from anywhere in the world via remote access. Systems are in place for shipping samples to and from Diamond Light Source. New users can borrow pucks and tools.
In situ data collection and sample characterization directly from crystals in their crystallization plates.
Control of the sample crystal's relative humidity with the aim of improving diffraction quality through improved packing of protein molecules constituting the lattice.
On-line and off-line UV-Vis spectroscopy of macromolecular samples to study enzyme reaction mechanisms and radiation damage.
Crystal reorientation to optimize data collection strategies for anomalous diffraction data collection and low symmetry spacegroups, or very long axis unit cells.
All beamlines are designated bio containment level 1. For containment level 2 samples beamlines I03 and I24 can be used and beamline I03 can be configured to run at biocontainment level 3.
High throughput and highly automated beamline for optimised MAD and SAD experiments. Capable of accepting CL3 type experiments on crystals of pathogens.
Wavelength: 0.5Å - 2.5Å
Detector: Pilatus3 6M
Variable focus from 5 to 100 microns, high throughput and highly automated beamline for optimised MAD and SAD experiments.
Beamsize: 10 x 5 - 110 x 100
Wavelength: 0.69Å - 2.07Å
Detector: Pilatus 6M-F
High throughput and highly automated fixed wavelength SAD beamline for macromolecular crystallography.
Beamsize: 60 x 50
Wavelength: 0.92Å (fixed)
Detector: Pilatus 6M-F
High throughput variable microfocus beamline for optimised MAD and SAD experiments on crystals down a few microns in size.
Beamsize: 5x5 - 50x40 (variable aspect ratio)
Wavelength: 0.62Å - 1.77Å
Detector: Pilatus3 6M
The Versatile Macromolecular X-tallography in-situ (VMXi) beamline will be an entirely automated facility for characterisation of, and data collection directly from, crystallisation experiments in situ.
VMXm is a micro/nanofocus Macromolecular Crystallography (MX) beamline aimed at atomic structure determination in cases where the production of significant quantities of protein material and crystals is problematic.
Energy: 7.0 - 25.0 keV
Wavelength: 0.57 - 2.0 Å
Detector: Pilatus 6M
The long-wavelength macromolecular crystallography beamline I23 is a unique facility for solving the crystallographic phase problem, using the small anomalous signals from sulphur or phosphorous which are present in native protein or RNA/DNA crystals. Additionally, anomalous difference Fourier maps can be used to locate sulphur and phosphorous positions to assist model building at low resolution and / or identify lighter atoms such as chlorine, potassium and calcium.
Energy: 2.1 – 11 keV
Wavelength: 1.1 – 5.9 Å
|Wavelength range (Å) ||0.6 - 2.3||0.69 - 2.07||0.92 (fixed)||0.7 - 2.0|
|Energy range (keV) ||5.2 - 21.0||6.0 - 18.0||13.5 (fixed)||6.4 - 20.0|
|Default settings (Å/keV)||0.98 / 12.7||0.98 / 12.66||0.92 / 13.53||0.97 / 12.8|
|Flux (ph/s) in full beam at default energy at 300 mA||1.7 x 1012||2.8 x 1011||9.0 x 1011||3.0 x 1012|
|Default beamsize (µm)||90 x 20||30 x 20||60 x 50||8 x 8|
|Full beam size at sample (µm)||90 x 20 90 x 30 100 x100 55 x 10||10 x 5 --> 110 x 100||60 x 50|| |
5 x 5 --> 50 x 40
|Available apertures (µm)|| |
20, 50, 100
|10, 20, 30, 50 and 70|
|Detectors (Pilatus type)|| |
|Number of unipucks / SPINE pins||30 / 480||37 / 592||37 / 592||37 / 592|
|Maximum samples / hour||30||30||30||30|
|Typical samples / hour||15 - 25||15 - 25||15 - 35||15 - 25|
Reserve access well ahead of your visit either at the links in the web page or via your local contact to facilities such as
Understand the visit timings you are scheduled for.
Refer to the manual for data collection possibilities –