Quick Guide to Using the MX Beamlines
Reserve access well ahead of your visit either at the links in the web page or via your local contact to facilities such as
Understand the visit timings you are scheduled for.
For remote experimenters read the remote access guidelines.
- For full remote access experiments register users and contact phone numbers for the visit and email your local contact and email@example.com with your expected number of pucks five working days before experiment.
- For mixed remote access (i.e. where a member of your team is on-site) ensure they will be available to change pucks.
- In both cases discuss your requirements for remote access with your local contact before your experiment.
For all experiments we highly recommend preparing your shipments in advance in ISPyB, identifying your pucks by the puck identifier (e.g. CPS-0024) and assigning samples at the same time. This allows pucks to be assigned to robot positions as loaded by either you or Diamond staff making them available in GDA immediately. For remote access this is a requirement rather than a recommendation to ensure reliable information transfer.
For UK users with Diamond registered dewars you can book your next working day shipping to us with Diamond’s DHL account. We recommend shipping your dewars three or more working days before your experiment to ensure arrival in time.
Register your PDB and sequences for your proteins in ISPyB. These can then be used automatically if you use default file names in GDA for all future visits in downstream processing such as XIA2, DIMPLE and MrBUMP and avoids the need to do this for each visit.
Refer to the manual for data collection possibilities –
- grid scans - find your crystals or the best diffracting region of a crystal and centre reliably
- line scans – minimise radiation damage, maximise resolution
- fluorescence data – find out if you have metals and what energy to use to exploit for phasing
- in-situ experiments - test crystal and collect data in trays
- dehumidifier HC1 - optimise diffraction by adjusting humidity
- data collections – optimise your data collections:
- inverse beam
- interleaved MAD
- washing and annealing crystals and other tools.
Read the message of the day in GDA for beamline status.
Refer to beamline specific manuals where appropriate.
Start your data back-up as soon as you can.
Load your pre-registered pucks and assign positions on the touch screen in the hutch (soon in all beamlines). Now start GDA and all your samples will be preloaded in the sample changer view (or press refresh to update if GDA was already running).
Use default file names in GDA to streamline experiments, avoiding the need to type information at each sample load, preventing mistakes and ensuring downstream processing uses the information you have provided in advance (PDB, sequence, space group).
Talk with your local contact about your experiment plans – we are here to help you solve structures.
Unload your last sample with the sample changer with sufficient time to ensure you have removed pucks from the sample changers before the end of your shift (unless you have discussed a time extension with the local contact).
Deregister your pucks in GDA or on the touch screen in the hutch as you remove them from the sample changer.
Finalise your backup disk and unmount cleanly in the data dispenser interface.
Diamond pays for sample shipping (2 dewars per shift) but for UK users only if you use our shipping agents via our webpages. No reimbursement of UK shipping will be made after August 2014 for shipping you arrange yourself.
You can reprocess your data afterwards on our cluster.