I04 is one of five macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9795 Å (12.658 keV) but it is tuneable over the wavelength range 0.69 - 2.066 Å (6 keV - 18 keV). On request it is also possible to work at a wavelength as low as 0.62 Å (20keV) with effective 1010 ph/s flux at the sample.
The beamsize can be focussed from 10 x 5 µm2 to 110 x 100 µm2 (h x v) across the available energy range. On request smaller beams in the vertical direction are possible. This will primarily depend on the working wavelength.
All MX beamlines use SPINE standard pins and Unipucks from cryocooled samples. The beamline can be accessed remotely.
The beamline is now equipped with the first Eiger2 X 16M detector which will enable faster data collection and new data collection protocols.
Latest generation Eiger2 X 16M detector on I04. More information here.
Smargon and new goniometer positioners installed - fast grid scans available.
We will be barcoding pucks from April 2017 onwards.
Data reprocessing now available in ISPyB. See here
New monochromator for I04. Big improvements, more to learn and deliver to users over coming months.
New remote access connection available
New BART robot installed on I04 - 37 pucks, 592 samples, 2.5x faster exchange times!
New focus options on I04. Variable beam size from 5 - 100 microns between 6 - 14 keV.
New web interface for ISPyB launched. For more information click here.
Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.
Loan pucks available upon request, check here
Test your network connection to Diamond with our SpeedTest
Crystal washer now available
I04 installed a Pilatus 6M-F detector in April 2013.
Remote access available on all MX beamlines at all times since January 2013
|Variable Focus Beam||✓|
|Fluorescence data collection||✓|
|Interleaved energy data collection|
|Inverse beam data collection|
|IMISX Capable for LCP in situ studies - discuss with staff before arrival ||✓|
|In situ goniometry for crystallisation plate ||✗|
|Automated strategy calculation||✓|
|Automated data processing||✓|
|Automated difference map calculation||✓|
|Automated molecular replacement||✓|
|Standard energy (Å / keV)||0.98 / 12.66|
|Flux at 12.66 keV (ph/s)||2.8 x 1011|
|Wavelength range (Å)||0.69 - 2.07|
|Energy range (keV)||6.0 - 18.0|
|Beam size at sample (µm)||10 x 5 --> 110 x 100|
|Detector||Eiger2 X 16M|
|Time to collect 180o data set (seconds)||15 +|
|No. of pins in dewar||592|
|Sample exchange time (seconds)||18|
|Guide to samples screened/hour (user centering)||30|
+ 0.1o per 0.008 second exposure 1800 frames (shutterless)
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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