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Macromolecular Crystallography
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In This Section

Sub Navigation
  • I03 Manual
    • Preparing for Beamtime
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      • Navigating in the GUI
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      • Load a Sample
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      • Centring Methods
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      • X-ray Centring
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Instruments by Science Group

Macromolecular
Crystallography
Soft Condensed
Matter
Imaging and
Microscopy
Biological
Cryo-Imaging
Magnetic
Materials
Structures and
Surfaces
Crystallography
Spectroscopy

I03 Contact

Beamline Phone Number:
+44 (0) 1235 778707

Acting Principal Beamline Scientist:
Dave Hall
Tel: +44 (0) 1235 778926 
E-mail: david.hall@diamond.ac.uk

I03 MX

Status: Operational

Beamsize: variable
Detector: Eiger2 XE 16M
Wavelength: 0.5 - 2.5 Å

In situ Multi-axis Goniometry Humidity Control Biocontainment Macromolecular Crystallography MAD: Multi Wavelength Anomalous Diffraction Remote Access X-ray Diffraction
  1. Instruments
  2. MX
  3. I03
  4. I03 Manual
  5. Data Collection Software
  6. X-ray Centring

X-ray Centring

Using X-ray grid scans to automatically centre your sample

You can use grid scans to automatically centre your sample. This method is very successful, even with LCP or ice covered crystals.

Manually triggered automatic X-Ray centring

For an already mounted sample, you can manually trigger X-Ray centring as follows: 

  • First, make sure your loop is roughly centred in the field of view.
  • Click the X-Ray button on the Align tab of the Alignment view in GDA.

The X-Ray centring process will take approximately 50s and you can view the grid scans via Synchweb. Bear in mind that the scoring results displayed in Synchweb do not reflect those from the automated X-Ray centring process, which is configured to avoid salt and ice related diffraction.

 

Automatic X-Ray centring as part of a data collection or screening operation

It is possible to request X-Ray centring when driving data collections for each new robot loaded sample. You can do this as follows:

  • Configure your desired data collection or screening settings from the Data Collection perspective.
  • Click the Sample ID (Select Samples and Run for screening) and choose the samples you want to collect with these settings.
  • At the bottom of the sample selection popup, make sure you choose the Auto X-Ray radio button as shown below.

  • For a Screening queue, clicking OK will start the queue automatically and X-Ray centre each new loaded sample prior to collecting screening images.
  • For a Data Collection, clicking OK will populate the Data Collection Input Table with a separate row for each sample. You can now choose Run All from the top of the form to collect every sample. 
  • X-Ray centring triggered in this fashion will only operate on a fresh sample load so don't load the first sample manually before starting the queue.

How it works

If you now load your sample, the routine puts the loop at the crosshair (Keyence Centring) and performs Loop centring to make sure the sample is in focus at all omega angles.

It then automatically carries out a two-dimensional grid scan covering the whole height of any object in view and extending from the furthermost tip to the edge of the visible image. This covers the entire loop and any protruding crystals and extends back along the neck towards the pin (we quite often find crystals along the neck of the loop). These grid scans use a smaller area of the detector for speed of processing and the beam size and exposure settings to be used are set by the beamline staff.

Each image from this grid scan is scored by the number of Bragg candidates using data to 5Å and the centre of mass is calculated from the results. The motors drive this position to the crosshair.

Afterwards, a finer, linear grid scan is performed perpendicularly to the two-dimensional one at the position where the crystal diffracts best. This is scored in the same fashion as before and again the best point is driven to the crosshair. The crystal is now centred.

At this point. data collection is automatically started. The whole process from loading the sample to the end of a 360 degree data collection with standard parameters takes approximately 2.0 min. 

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