I03 Manual

Reserve access well ahead of your visit either at the links in the web page or via your local contact to facilities such as

• in-situ experiments
• dehumidifier HC1
• biocontainment
• microspectroscopy
• xenon gas cell

Check the Diamond MX web pages and individual beamline pages for updates, manuals and contact details prior to your experiment.

Register your shipping dewars with Diamond to use our shipping account and help with tracking.

Always purchase and use SPINE pins and Unipucks to mount and ship your samples. Non-SPINE standard pins cause robots to crash and damage to equipment: you lose beamtime, we lose sleep.

For full remote access experiments we prefer pucks to arrive in the shelved shipping cane or alternatively the puck shipping canister. Please avoid the narrow canisters where possible.

For remote experimenters read the remote access guidelines.

• For full remote access experiments register users and contact phone numbers for the visit and email your local contact and [email protected] with any issues.
• In both cases discuss your requirements for remote access with your local contact before your experiment.

For all experiments we highly require you to prepare your shipments in advance in ISPyB, identifying your pucks by the puck identifier (e.g. CPS-0024) and assigning samples at the same time. This allows pucks to be assigned to robot positions when loaded by Diamond staff.

For UK users with Diamond registered dewars you can book your next working day shipping to us with Diamond’s DHL account. We recommend shipping your dewars three or more days before your experiment to ensure arrival in time.

Register your PDB and sequences for your proteins in ISPyB. These can then be used automatically if you use default file names in GDA for all future visits in downstream processing such as XIA2, DIMPLE and MrBUMP and avoids the need to do this for each visit.

Refer to the manual for data collection possibilities –

• grid scans - find your crystals or the best diffracting region of a crystal and centre reliably
• line scans – minimise radiation damage, maximise resolution
• fluorescence data – find out if you have metals and what energy to use to exploit for phasing
• in-situ experiments - test crystal and collect data in trays
• dehumidifier HC1 - optimise diffraction by adjusting humidity
• data collections – optimise your data collections:
  • strategies
  • multi axis goniometry
• washing and annealing crystals and other tools.

Read the message of the day in GDA for beamline status.

Refer to beamline specific manuals where appropriate.

Start your data back-up as soon as you can.

Load your pre-registered pucks and assign positions on the touch screen in the hutch (soon in all beamlines). Now start GDA and all your samples will be preloaded in the sample changer view (or press refresh to update if GDA was already running).

Use default file names in GDA to streamline experiments, avoiding the need to type information at each sample load, preventing mistakes and ensuring downstream processing uses the information you have provided in advance (PDB, sequence, space group).

Monitor your results from our automatic software pipelines in ISPyB.

Talk with your local contact about your experiment plans – we are here to help you solve structures.

Unload your last sample with the sample changer with sufficient time to ensure you have finished before your session is over. Contact Diamond staff if you require extra time, but this is depending on beamline availability.

Finalise your backup disk and unmount cleanly in the data dispenser interface.

Request return shipment of your dewar.

Diamond pays for sample shipping for UK and EU users, but only if you use our shipping agents via ISPyB.

You can reprocess your data afterwards on our cluster.

Acknowledge beamline access.