Registering Samples

To register samples, it is first necessary to create a new shipment, and detail information on all proteins.
 
  1. Create Shipment
  2. Create Protein
  3. Register Samples

Once a Shipment has been created click the "Add Container" button on a dewar in the shipment

Add Container

This will take you to the register container page. Here you can register samples for this puck.

Firstly you need to select the puck from the drop down at Registered Container. After that each sample needs to be given a protein acronym and sample name as a minimum. Barcode and spacegroup can also be specified if needed. The "+" icon can be used to clone a sample in a similar mannar to the GDA client.

creating a container

When a protein acronym and sample name has been specified ISPyB considers the sample to be valid and shows this as a green circle in the puck graphic. You can navigate to a particular sample position by clicking on the puck graphic

Ideally the protien should be registered, to gain best use of automated pipelines a pdb and sequence are required. It is possible to add a new protein acronym by typing into the protein acronym box and press Tab.

New Protein

You will be prompted to confirm the new protein addition

Confirm New Protein

Click "Ok" and the new protein will be created

To run automated phasing pipelines (Fast EP or Big EP)  the anomalous scatterer must be supplied in the sample registration. 

Adding an Anomalous Scatterer

Finally to save your new puck click "Add Container" at the bottom of the page

Using the old ISPyB Excel Spreadsheet

Although not recommended, ISPyB2 supports the old style Excel Spreadsheet. First fill in the spreadsheet as you would usually.

Populated Spreadsheet

Now select the current spreadsheet using CTRL-A (CMD-A on Mac) to select the spreadsheet. Depending on your location in the sheet you may need to press this twice.

Selected Spreadsheet

Now press CTRL-C (CMD-C on Mac) to copy the contents of the selection to the clipboard. Now go back to ISPyB2 and click the "Paste from Spreadsheet" button

 

Paste from Spreadsheet

This will show a popup. In this popup press CTRL-V (CMD-V on Mac) to paste the contents of the clipboard, then click "Insert"

Popup Pasted

The copied samples will now be inserted into the current puck

Pasted Samples

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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