Advanced Photon Source (APS) Macromolecular Crystallography users have been allocated access to Diamond during the APS Upgrade installation (starting April 2023) and beamline commissioning period. Users from the general user program, LS-CAT and SER-CAT have applied via APS for access to four high-throughput MX beamlines at Diamond (I04, I24, I03, and I04-1).
Another call will go out to the APS users for the time later in the upgrade installation.
Two training sessions were run covering administration and data collection respectively, the talks and slides from these are below.
A summary of Macromolecular crystallography at Diamond.
An overview of roles within a Diamond Proposal.
Slides: Accessing Diamond
An explanation of how visits are scheduled. Most MX visits are scheduled once samples arrive at Diamond. Visits can be rebooked if preferred, especially for training.
Slides: Scheduling remote visits
Summary of sample holders used; for normal MX experiments Diamond uses Unipucks and SPINE pins.
Slides: Sample holders
GDA is used for interactive remote data collection.
Slides: Data Retrieval
The long-wavelength macromolecular crystallography beamline I23 is a unique facility for solving the crystallographic phase problem, and/or identifying and locating lighter atoms of biological relevance such as S, P, Cl, K and Ca.
Access is not provided directly by this call, but rapid access to the beamline can be arranged. Initially please get in touch with Armin Wagner the principal beamline scientist of I23.
Crystallographic fragment screening is enabled throughout the XChem facility at Diamond. The talk gives a brief summary of how the experiment works, and what is needed to take a crystal system through XChem.
Academic access to XChem is via a standard 6-monthly proposal call system within Diamond, to discuss viability contact Frank von Delft and/or Daren Fearon. For proprietary access, contact Alex Dias from the Industrial Liaison Office.
Slides: Brief Introduction to XChem
The membrane protein laboratory is a facility that supports membrane protein structural biology projects at any stage from the high-throughput cloning of constructs all the way through to assisting with structural solutions.
Slides: Membrane Protein Laboratory
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