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The Titan Krios are fully automated cryo-electron microscopes from Thermo Fisher Scientific, designed for rapid, stable, high-resolution data collection on frozen-hydrated samples.
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) data can be collected automatically on all four Krios microscopes.
All Krios microscopes are equipped with the latest generation Ametek-Gatan Bio-quantum-K3 detectors, as well as the Thermo Fisher Scientific Falcon III detectors.
The Talos Arctica is Thermo Fisher Scientific's new generation fully automated 200 kV FEG electron microscope. It is equipped with an autoloader system that permits loading of up to twelve specimens, automated data collection at liquid nitrogen temperature, and features a Constant-PowerTM objective lens for optimal contrast/resolution balance.
The Scios and Aquilos cryoFIB/SEMs (focused ion beam/scanning electron microscopes) allow for nanoscale milling of frozen hydrated biological specimens.
These instruments mainly produce electron transparent lamellae but are also capable of directly imaging cellular features. Lamella are analysed using cryo-electron tomography (CryoET) which can reveal high-resolution biological structures in their close-to-native environment and direct imaging can provide cellular context.
These powerful techniques have allowed researchers to move toward a molecular scale understanding of living systems.
The cryoCLEM is a dedicated cryo-fluorescence microscope, designed to maintain cryo-conditions for specimen handling. It provides high resolution imaging to enable the user to target the regions of interest for the correlative process.
The Correlative Light and Electron Microscopy (CLEM) process includes the extra information provided by the fluorescence signal and guides the cryoEM tilt series collection, making the data acquisition much more accurate and efficient.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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