Tel: +44 (0) 1235 56 7480
E-mail: EBICadmin@diamond.ac.uk
Peijun Zhang
Tel: +44 (0) 1235 77 8878
E-mail: peijun.zhang@diamond.ac.uk
Daniel Clare
Tel: +44 (0) 1235 56 7501
E-mail: daniel.clare@diamond.ac.uk
Yuriy Chaban
Tel: +44 (0) 1235 77 8207
E-mail: yuriy.chaban@diamond.ac.uk
Karen Davies
Tel: +44 (0) 1235 77 8057
E-mail: karen.davies@diamond.ac.uk
Kyle Morris
Tel: +44 (0) 1235 39 4094
E-mail: kyle.morris@diamond.ac.uk
The Titan Krios are fully automated cryo-electron microscopes from Thermo Fisher Scientific, designed for rapid, stable, high-resolution data collection on frozen-hydrated samples.
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) data can be collected automatically on all four Krios microscopes.
All Krios microscopes are equipped with the latest generation Ametek-Gatan Bio-quantum-K3 detectors, as well as the Thermo Fisher Scientific Falcon III detectors.
Thermo Fisher Scientific's EPU software, with aberration free image shift data collection (AFIS), is primarily used for single particle data collection (SPA) at eBIC. SerialEM is also available on all four Krios microscopes for optimal cryo-ET data collection.
All Krios are equipped with Volta style phase plates, greatly boosting low-resolution contrast, facilitating cryo-EM on small proteins and cryo-ET applications.
Benefits for structural biology
Who should apply for use?
eBIC particularly encourage applications from users with frozen-hydrated specimens that have been optimised in their home laboratories, and which would greatly benefit from the increased data quality and throughput the Krios offers.
Loading samples
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
Copyright © 2022 Diamond Light Source
Diamond Light Source Ltd
Diamond House
Harwell Science & Innovation Campus
Didcot
Oxfordshire
OX11 0DE
Diamond Light Source® and the Diamond logo are registered trademarks of Diamond Light Source Ltd
Registered in England and Wales at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom. Company number: 4375679. VAT number: 287 461 957. Economic Operators Registration and Identification (EORI) number: GB287461957003.