Tel: +44 (0) 1235 56 7480
Tel: +44 (0) 1235 77 8878
Tel: +44 (0) 1235 56 7501
Tel: +44 (0) 1235 77 8207
Tel: +44 (0) 1235 77 8057
Tel: +44 (0) 1235 56 7537
The Titan Krios is a fully automated cryo-electron microscope from Thermo Fisher Scientific, designed for rapid, stable, high-resolution data collection on frozen-hydrated samples.
Four Titan Krios microscopes are available at eBIC for academic users. Krios I, II, & IV are equipped with the latest generation Ametek-Gatan Bio-quantum-K3 detectors and energy filter. Krios III is equipped with the Thermo Fisher Scientific Falcon 4i detector & Selectris X energy filter.
Cryogenic electron microscopy (cryo-EM) and cryogenic electron tomography (cryo-ET) data can be collected automatically on all four Krios microscope.
Thermo Fisher Scientific's EPU and Tomography software, with aberration-free image shift data collection (AFIS), are primarily used for single particle analysis (SPA) and tomographic data collection at eBIC, respectively. SerialEM is also available on all four Krios microscopes to trained users who can operate SerialEM independently.
All Krios microscopes are equipped with Volta style phase plates, greatly boosting low-resolution contrast, facilitating cryo-EM on small proteins and cryo-ET applications.
The fifth Krios is a dedicated microscope for use by commercial clients. As part of eBIC for Industry, it provides an important resource for structure-based drug discovery, for antibody and vaccine research, and to progress the study of nanotechnologies and nanomedicines.
Click on the buttons below for further details.
Benefits for structural biology
Who should apply for use?
eBIC particularly encourage applications from users with frozen-hydrated specimens that have been optimised in their home laboratories, and which would greatly benefit from the increased data quality and throughput the Krios offers.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
Copyright © 2022 Diamond Light Source
Diamond Light Source Ltd
Harwell Science & Innovation Campus
Diamond Light Source® and the Diamond logo are registered trademarks of Diamond Light Source Ltd
Registered in England and Wales at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom. Company number: 4375679. VAT number: 287 461 957. Economic Operators Registration and Identification (EORI) number: GB287461957003.