Biological Cryo-Imaging
eBIC
Macromolecular
Soft Condensed 
Imaging and
Biological
Magnetic
Structures and
Crystallography 
Spectroscopy 
Titan Krios Specifications
Krios I
-
Krios II
-
+
Krios III
-
+
Krios IV
-
+
| Accelerating Voltages | 80, 120, 200, 300 kV |
| Electron Source | Schottky X-FEG |
| Tilt Range | ± 70° |
| C1 Apertures | 2000, 70, 50 and 30 µm |
| C2 Apertures | 150, 100, 70 and 50 µm |
| Objective Apertures | 100, 70 µm and phase plate assembly |
| Operating Temperature | ~80 K |
| Information Limit at 300kV at 0° / ±70° tilt | 0.14 nm and 0.23 nm |
| Cs | 2.7 mm |
| Tomogram acquisition time | ~45 mins |
| Number of cartridges | 12 |
| Sample exchange time | ~3 mins |
| Retractable bottom mount detector | FEI Falcon III. 4096 x 4096, 14 µm pixel. 38/39 fps max frame rate. DQE@ NF/2 > 0.5 |
| Imaging filter and detector | Gatan Quantum K2 Summit 3838 x 3710 pixels, 5 µm pixel 40 fps max frame rate DQE@NF/2 = ~0.56 |
| Acquisition software | EPU 1.9.1, Tomo 4.3.1, SerialEM 3.7 |
| Accelerating Voltages | 80, 120, 200, 300 kV |
| Electron Source | Schottky X-FEG |
| Tilt Range | ± 70° |
| C1 Apertures | 2000, 70, 50 and 30 µm |
| C2 Apertures | 150, 100, 70 and 50 µm |
| Objective Apertures | 100, 70 µm and phase plate assembly |
| Operating Temperature | ~80 K |
| Information Limit at 300kV at 0° / ±70° tilt | 0.14 nm and 0.23 nm |
| Cs | 2.7 mm |
| Tomogram acquisition time | ~45 mins |
| Number of cartridges | 12 |
| Sample exchange time | ~3 mins |
| Retractable bottom mount detector | FEI Falcon III. 4096 x 4096, 14 µm pixel. 38/39 fps max frame rate DQE@ NF/2 > 0.5 |
| Imaging filter and detector | Gatan Quantum K2 Summit 3838 x 3710 pixels, 5 µm pixel 40 fps max frame rate DQE@NF/2 = ~0.56 |
| Acquisition software | EPU 1.9.1, Tomo 4.3.1, SerialEM 3.7 |
| Accelerating Voltages | 80, 120, 200, 300 kV |
| Electron Source | Schottky X-FEG |
| Tilt Range | ± 70° |
| C1 Apertures | 2000, 70, 50 and 30 µm |
| C2 Apertures | 150, 100, 70 and 50 µm |
| Objective Apertures | 100, 70 µm and phase plate assembly |
| Operating Temperature | ~80 K |
| Information Limit at 300kV at 0° / ±70° tilt | 0.14 nm and 0.23 nm |
| Cs | 2.7 mm |
| Tomogram acquisition time | ~45 mins |
| Number of cartridges | 12 |
| Sample exchange time | ~3 mins |
| Retractable bottom mount detector | FEI Falcon III. 4096 x 4096, 14 µm pixel. 38/39 fps max frame rate DQE@ NF/2 > 0.5 |
| Imaging filter and detector | Gatan Quantum K2 Summit 3838 x 3710 pixels, 5 µm pixel 40 fps max frame rate DQE@NF/2 = ~0.56 |
| Acquisition software | EPU 1.9.1, Tomo 4.3.1, SerialEM 3.7 |
| Accelerating Voltages | 80, 120, 200, 300 kV |
| Electron Source | Schottky X-FEG |
| Tilt Range | ± 70° |
| C1 Apertures | 2000, 70, 50 and 30 µm |
| C2 Apertures | 150, 100, 70 and 50 µm |
| Objective Apertures | 100, 70 µm and phase plate assembly |
| Operating Temperature | ~80 K |
| Information Limit at 300kV at 0° / ±70° tilt | 0.14 nm and 0.23 nm |
| Cs | 2.7 mm |
| Tomogram acquisition time | ~45 mins |
| Number of cartridges | 12 |
| Sample exchange time | ~3 mins |
| Retractable bottom mount detector | FEI Falcon III 4096x4096, 14µm pixel. 38/39 fps max frame rate DQE@ NF/2 > 0.5 |
| Imaging filter and detector | Gatan Quantum K2 Summit 3838x3710 pixels, 5 µm pixel 40 fps max frame rate DQE@NF/2 = ~0.56 |
| Acquisition software | EPU 1.9.1, Tomo 4.3.1, SerialEM 3.7 |