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Yes you will need to show that you have good quality cryo-EM grids prepared, with cryo images and 2D classes showing clear secondary structure.
Each 48 hour session is designed for data collection on one/two samples and is normally enough to enable a high resolution structure determination. Sessions are designed for collection of a full data set from an optimised sample. We recommend bringing 8 grids and we will load 4 of these. You could bring a back up sample, provided:
Rapid proposals generally provide 2 days of microscope time, which should be allocated within 6 weeks of the application being received.
BAG proposals provide large amounts of time for an institution/group of users over a 2 year period with allocated time starting 6 months after acceptance.
*Every 6 months progress reports must be submitted for the BAG to continue.
Every 3 months (1st January, April, July and October).
Every 6 months (1st of April and 1st October).
No you will need to make a new application.
There is no minimum requirement for the number of researchers within a BAG, applications will be assessed on the number and quality of the scientific projects contained within the proposal.
Krios I, II and IV are equipped with Gatan BioQuantum K3 imgaing filter and detector; Krios 3 is equipped with Thermo Fisher Scientific Selectris X imaging filter and Falcon 4 detector. Please see instruments page for current set up of individual microscopes or contact your Local Contact for information.
We do not support portable hard drives and recommend Globus/FTP be used to transfer data to your home institution.
Please see the remote access and analysis user guide for more details.
If you want to add more images to an existing dataset please advise us and we will try and schedule your microscope time accordingly.
Details are listed in the eBIC web page Publications acknowledgement.
"(We acknowledge) Diamond for access and support of the Cryo-EM facilities at the UK national electron bio-imaging centre (eBIC), proposal EM####, funded by the Wellcome Trust, MRC and BBSRC.”
Please be aware that eBIC requires that ALL experimental details, including the use of phase plates, are submitted via UAS a minimum of 2 weeks in advance of your microscope session. eBIC regrets that we may not be able to accommodate requests that are received after this time.
Yes, but session setup support is only available during working hours on Friday.
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