eBIC: Electron Bio-Imaging Centre

Deadline for proposal submission:
17:00hrs (BST) on Thursday 30^th September 2021

Call will provide access to MicroED for a limited number of selected user proposals (Academic users only).

Initially only on-grid MicroED data collection with a Ceta-D detector and EPU-D data collection software on the Talos Arctica (200 keV) will be available.

Further details are available here (download).

 CryoFIB user program

Submit your proposal

• For lamella preparation on frozen cells and microcrystals

• On-grid FIB milling only

• Rapid access

For more information please see here (download).

We are pleased to announce a commissioning call for researchers to access the chameleon cryo-EM freezing device (application) developed commercially by SPT Labtech. This forms part of a collaboration between Diamond, the Rosalind Franklin Institute and SPT Labtech with the view to providing a platform for beta-testing of the chameleon by the wider user community that will aid further commercialisation of the system by SPT Labtech.

The chameleon uses piezo electric inkjet dispensing technology to apply nanolitre volumes of protein solution to self-wicking cryo-EM grids.

Please select the Rapid access route and the Talos instrument when you submit your proposal and also add that this is specifically for use with the chameleon.

• Proposals are also open to existing BAG PIs.
• Proposals will be reviewed by eBIC staff with a view to schedule beamtime from December/January.
• Please follow this link for details about requirements and visit structure (download).
• For inquiries please contact daniel.clare@diamond.ac.uk.

Post-Doctoral Research Associate - Correlative Microscopy

We are seeking a highly motivated PDRA to develop and apply a multi-scale and correlative microscopy workflow to understand the germicidal effects of anti-microbial agents on microorganisms. This is a collaboration between Procter & Gamble and the Diamond Light Source.

You will use optical microscopes (cryoSIM and Leica cryoCLEM), cryo-Soft X-ray Tomography (SXT), with Scios/Aquilos cryoFIB/SEM instrument and Krios microscopes at Diamond and set up novel experiments to investigate how individual antimicrobial agents (AMA) affect the cell (e.g. peroxidation, lipid plasticisation, chelation of multivalent cations bound to the lipids and permeation of small molecules through it) and to identify the best combination for fast kill.

Read more here... Post-Doctoral Research Associate - Correlative Microscopy