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The electron Bio-Imaging Centre (eBIC) was established at Diamond Light Source following the award of a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC).
eBIC provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for single particle analysis, electron tomography and electron diffraction. The location of eBIC enables scientists to combine their techniques with many of the other cutting-edge approaches that Diamond offers.
Currently eBIC houses five Titan Krios microscopes, a Talos Arctica, two Glacios, a Scios and an Aquilos cryo-FIB/SEM, and a Leica CryoCLEM.
Access to eBIC is available and currently operating under general beamline guidelines.
We are able to offer remote access, unattended automated data collection (currently only available: data collection using TFS EPU and TFS Tomography) and sample mail-in services for all research areas.
COVID-19 research should be requested through rapid access proposals following these instructions.
NOTE: Diamond EHC (+44 (0)1235 778787) are available for support, providing 24-hour coverage during the week when the beamline is on. Over the weekend EHC coverage is for 12hours only from 7am-7pm (Sat & Sun).
Postdoctoral Research Associate - Bacterial Chemotaxis
We now have a PDRA position available in Professor Zhang’s lab at eBIC on a 2 year fixed term contract. The position is funded by a 5-year ERC AdG grant award on “Molecular choreography of bacterial chemotaxis signalling”.
We are seeking highly motivated scientist to conduct high-resolution structural analysis of chemosensory arrays in distinct conformational states, in vitro and in vivo in native cells, as well as in time-resolved signalling states upon chemical stimulation, using cryoET and subtomogram averaging.
Chemotaxis - a ubiquitous biological behaviour enabling the movement of a cell or organism toward or away from chemicals - has severed as a paradigmatic model for the study of cellular sensory signal transduction and motile behaviour. This project is focused on understanding the underlying molecular mechanisms of chemosensory array assembly, activation by its stimulus and high cooperativity, by determining the precise interactions between the signalling components within in vitro reconstituted array systems and in native E. coli cells. We will use a combination of innovative and cutting-edge cryoEM/ET methods, including high-resolution cryoET subtomogram averaging, time-resolved conformational changes during signalling, and computational modelling of dynamics, as well as in vivo functional assays, to investigate the structural and dynamical mechanisms underlying signal transduction, propagation, and regulation in the chemosensory array.
Read more here...Postdoctoral Research Associate - Bacterial Chemotaxis
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