autoPROC(STARANISO)

autoPROC is an autoprocessing pipeline from Global Phasing. It combines several 3rd party programs (XDS, AIMLESS, XSCALE, POINTLESS, MOSFLM) with original tools to provide a complete data processing workflow.

Some datasets exhibit anisotropic diffraction, meaning that diffraction strength and resolution limit vary with direction in reciprocal space. In practice, this appears as higher-resolution and/or stronger diffraction along certain directions, and weaker, lower-resolution data along others.

Strong anisotropy can negatively affect merging statistics and map quality if treated as isotropic data (see here).

To address this, the program STARANISO, is run after autoPROC. STARANISO estimates the directional dependence of diffraction and applies anisotropic scaling and truncation. This can improve the overall quality of the processed data. Both the autoPROC processing results and the subsequent STARANISO processing are available as distinct tabs in ISPyB.

Documentation for autoPROC can be found here: https://www.globalphasing.com/autoproc/

If your publication uses data processed with the autoPROC and STARANISO pipeline, please cite the following references:

• Vonrhein, C. "Data processing and analysis with the autoPROC toolbox" Acta Cryst. D67 (2011): 293-302.
• Vonrhein, C. "Advances in automated data analysis and processing within autoPROC, combined with improved characterisation, mitigation and visualisation of the anisotropy of diffraction limits using STARANISO" Acta Cryst. A74 (2018).

If you deposit a structure in the PDB which is determined from data processed with the autoPROC pipeline and the STARANISO analysis, please make sure both autoPROC and STARANISO are included in the software category.