Learning Resources

There is a wealth of information on cryo-EM in the community mailing lists of 3DEM and CCPEM which remain very active. Generally you may find answers to very specific technical and academic questions, as well as broader high-level discussions.

There are otherwise lots of resources on the internet that aim to provide training and education in cryo-EM. This page aims to collate some of the resources that are available to give a helpful starting point to newcomers and or refresher for experienced cryo-EM structural biologists alike. It is not intended to be an exhaustive reference list.

When using these resources, the reader is encouraged to consider when the resource was created. There is always something to be learned but cryo-EM techniques have and will continue to develop rapidly, so when you are at the microscope in your own institute do follow the advice and protocols that are in place locally.

Microscope locations worldwide
Google map

Thermo Fisher Scientific resources
Interactive cryo-EM technique overview

How to make a microscope (YouTube - suitable for the public)

Caltech Jensen courses
https://cryo-em-course.caltech.edu/overview

LMB-MRC courses
https://www2.mrc-lmb.cam.ac.uk/research/scientific-training/electron-microscopy

Birkbeck courses
2019: course resources
2023: course webpage

Thermo Fisher Scientific resources
Cryo-EM University

Media rich resources
Cryo-EM 101

JoVE: Single Particle Cryo-Electron Microscopy: From Sample to Structure

Fundamental Tutorials in Transmission Electron Microscopy
http://rodenburg.org

Transmission Electron Microscope Technical Diagrams
https://www.globalsino.com/

General:

Quick tutorial: The Linux command line:
https://ubuntu.com/tutorials/command-line-for-beginners#1-overview

Course: General introduction & computational skills:
https://software-carpentry.org/

Linux, shell, bash:

Quick tutorial: Bash commands:
https://www.datacamp.com/tutorial/shell-commands-data-scientist

Cheat sheet: Linux command line:
https://cheatography.com/davechild/cheat-sheets/linux-command-line/

Cheat sheet: awk command:
https://cheatography.com/tme520/cheat-sheets/awk-english/

Course: Introduction to bash scripting:
https://www.datacamp.com/courses/introduction-to-bash-scripting

Python:

Cheat Sheet: Python & data science:
https://www.datacamp.com/cheat-sheet