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Instruments by Science Group

Macromolecular
Crystallography
Soft Condensed
Matter
Imaging and
Microscopy
Biological
Cryo-Imaging
Magnetic
Materials
Structures and
Surfaces
Crystallography
Spectroscopy

Contact

Admin enquiries

Tel: +44 (0) 1235 56 7480 
E-mail: EBICadmin@diamond.ac.uk

Science enquiries

Peijun Zhang
Tel: +44 (0) 1235 77 8878
E-mail: peijun.zhang@diamond.ac.uk

Daniel Clare
Tel: +44 (0) 1235 56 7501
E-mail: daniel.clare@diamond.ac.uk

Yuriy Chaban
Tel: +44 (0) 1235 77 8207
E-mail: yuriy.chaban@diamond.ac.uk

Karen Davies
Tel: +44 (0) 1235 77 8057
E-mail: karen.davies@diamond.ac.uk

Training enquiries

Kyle Morris
Tel: +44 (0) 1235 56 7537
E-mail: kyle.morris@diamond.ac.uk

Science Group Leader

Martin Walsh

Email: martin.walsh@diamond.ac.uk
Tel: +44 (0)1235 778518

Funded by a grant from

Wellcome Trust

MRC

BBSRC

eBIC

Status: Operational

Accelerating Voltages: 3-30, 200 & 300 kV

Cryo-EM: Cryo Electron Microscopy Cryo-ET: Cryo Electron Tomography Imaging Electron Microscope Electron Crystallography FIBSEM: Cryo Focused Ion Beam Scanning Electron Microscopy Microscopy
  1. Instruments
  2. Biological Cryo-Imaging
  3. eBIC
  4. FAQ

FAQ

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    Yes you will need to show that you have good quality cryo-EM grids prepared, with cryo images and 2D classes showing clear secondary structure.

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    Each 48 hour session is designed for data collection on one/two samples and is normally enough to enable a high resolution structure determination.  Sessions are  designed for collection of a full data set from an optimised sample. We recommend bringing 8 grids and we will load 4 of these. You could bring a back up sample, provided:

     

    • you appreciate you will have to choose which sample to collect from on the day, and this extra deliberation will take up valuable data collection time
       
    • the experimental methods, and therefore microscope set up, would be the same for the second sample
       
    • the second sample was also in your agreed proposal
       
    • you have entered the sample in the ERA and it has been approved (please allow 2 weeks)

     

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    Rapid proposals generally provide 2 days of microscope time, which should be allocated within 6 weeks of the application being received.

     

    BAG proposals provide large amounts of time for an institution/group of users over a 2 year period with allocated time starting 6 months after acceptance.

     

    *Every 6 months progress reports must be submitted for the BAG to continue.

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    Every 3 months (1st January, April, July and October).

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    Every 6 months (1st of April and 1st October).

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    No you will need to make a new application.

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    There is no minimum requirement for the number of researchers within a BAG, applications  will be assessed on the number and quality of the scientific projects contained within the proposal.

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    Krios I, II and IV are equipped with Gatan BioQuantum K3 imgaing filter and detector; Krios 3 is equipped with Thermo Fisher Scientific Selectris X imaging filter and Falcon 4 detector. Please see instruments page for current set up of individual microscopes or contact your Local Contact for information.

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    Krios III.

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    We do not support portable hard drives and recommend Globus/FTP be used to transfer data to your home institution.

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    Please see the remote access and analysis user guide  for more details.

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    If you want to add more images to an existing dataset please advise us and we will try and schedule your microscope time accordingly.

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    "(We acknowledge) Diamond for access and support of the Cryo-EM facilities at the UK national electron bio-imaging centre (eBIC), proposal EM####, funded by the Wellcome Trust, MRC and BBSRC.”

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    Please be aware that eBIC requires that ALL experimental details, including the use of phase plates, are submitted via UAS a minimum of 2 weeks in advance of your microscope session. eBIC regrets that we may not be able to accommodate requests that are received after this time.

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    Yes, but session setup support is only available during working hours on Friday.

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