Tel: +44 (0) 1235 56 7480
Tel: +44 (0) 1235 77 8878
Tel: +44 (0) 1235 56 7501
Tel: +44 (0) 1235 77 8207
Tel: +44 (0) 1235 77 8057
Tel: +44 (0) 1235 56 7537
Tel: +44 (0)1235 778518
eBIC is welcoming a limited number of users back on-site during January-April 2023. Onsite access will be prioritised based on technique used. Order of priority is:
To comply with room CO2 limits, only two users per microscope will be allowed. For permission to attend on-site, please email requests to firstname.lastname@example.org at least two weeks before the scheduled session with an explanation of why an on-site visit is essential.
We strongly recommend users continue to use the ISPyB system for Sample Shipping. Users who do not use ISPyB shipping are solely responsible for their sample arriving and leaving eBIC. Only dry shippers entered into ISPyB will be checked and topped up with liquid nitrogen. All samples must be entered into the UAS and have an approved ERA before they arrive on site.
The electron Bio-Imaging Centre is located
on-site at Diamond, near the synchrotron.
electron Bio-Imaging Centre (eBIC)
Diamond Light Source
Harwell Science and Innovation Campus
Directions to Diamond itself can be found here.
For all microscopes: If users bring samples to site, please arrive at eBIC/i14 building entrance (opposite cafeteria) at 9am. If samples, shipped, please arrive at 11am. Please bring your user badge if you have been issued with one.
If you need assistance please contact the Experimental Hall Coordinators on:
+44 (0)1235 77 8787.
Essential user actions prior to on-site visit:
Key points during data collection setup:
Movie output format should be 'non-gain normalised LZW compressed tif'.
Fast-acquisition is recommended for most standard SPA setup as it uses AFIS allowing multiple shots per hole and per stage movement leading to higher data collection rates.
Our recommended setup with K3 cameras is super-resolution with 2-fold binning as this generates fastest data collection rates. To offset potential aliasing, it is recommended to collect one mag higher than required. (e.g., If targeting 81k (1.06 Å/px), use 105k (0.83 Å/px) instead.
For problems outside 9am-5pm (UK time) during the week when the beamline is on, and on weekends (7am-7pm), users must call Diamond EHCs on +44 (0) 1235 77 8787 and inform them:
- they are a user running a remote session on a Krios electron microscope.
- provide the EHC with their local contact's name.
The Diamond EHC will make best efforts to get in touch with eBIC staff, who will attempt to address the problem.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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Diamond Light Source Ltd
Harwell Science & Innovation Campus
Diamond Light Source® and the Diamond logo are registered trademarks of Diamond Light Source Ltd
Registered in England and Wales at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom. Company number: 4375679. VAT number: 287 461 957. Economic Operators Registration and Identification (EORI) number: GB287461957003.