Instruct-ERIC Soft X-ray Tomography workshop

Sep 11. till Sep 15.

Instruct-ERIC Soft X-ray Tomography workshop (2023)

Save the date for the upcoming Instruct-ERIC Soft X-ray Tomography workshop.

11/09/20232023-09-11T12:00:00 - 15/09/20232023-09-15T16:30:00
12:00 - 16:30
Diamond Light Source
Apply here


Instruct-ERIC workshop on imaging cells using correlative soft X-ray tomography at cryogenic temperatures

Soft X-ray tomography (SXT) is fast becoming an established and necessary imaging technology for the life sciences catering for the capture of high-resolution (25nm) ultrastructural information in fully hydrated cell populations under native conditions. The method can be used for same-sample correlative imaging alongside technique such as fluorescence imaging/cryo-imaging, X-ray scanning microscopy and cryo-electron tomography. 

This workshop is intended for graduate students and early-career researchers interested in gaining a deeper understanding and hands-on experience imaging cells in 3D using Soft X-ray Tomography at cryogenic temperatures (cryoSXT). The workshop will consist of a mixture of lectures and practicals that will demonstrate all aspects of the imaging pipeline at our beamline B24 from sample preparation to data acquisition and analysis. 

On behalf of Diamond Light Source and the biological cryo-imaging B24 beamline, we invite you to apply to this Instruct-ERIC workshop. 

CryoSXT has an ever-widening user-base and the requirement for investigator independence and technique ownership often relies on their ability to prepare good samples and to quickly extract meaningful information from the data acquired. 

The workshop seeks to: 

  • Guide you through each stage in the experimental workflow from sample preparation to data analysis with a combination of practical experiments and theory lectures delivered by scientists who pioneered and championed cryoSXT. 
  • Educate about best practices, including the identification of decision points and common pitfalls. 
  • Teach you to collect and analyse data from cryopreserved samples imaged using a structures illumination microscope (cryoSXT). 

This workshop will be delivered on-site and in-person and spaces are limitedapplicants who are not selected for the worshop are invited to join online for the lecture component via Zoom link, but registration is still required (There will be no registration fee for online attendance). 

Application and registration process

Applications are now open and places are strictly limited to 12 academic delegates. Places will be allocated on the grounds of merit and anticipated benefit. Our registration will be closing on 2nd August 2023 at 23:30 (BST)

All applicants will be informed on the result of their application first week in August. If your application is successful, there will be a £100 fee to attend the event, in person, at Diamond Light Source. Whilst places to this workshop are limited. Applicants who are not selected for the workshop are invited to join online for the lecture component. 

Course dates: 11th to 15th September 2023 (Draft agenda below)

  • July 2023 - Applications Open 
  • 2nd August 2023 at 23:30 (BST) - Applications close 
  • Early August 2023 - Successful selection announcement 

Diamond will cover the costs of accommodation and all meals and refreshments, including local transport to-and-from the synchrotron.

Attendees will be required to cover the cost of their own transport to and from the workshop. 

Monday 11th September

Monday 11th September 
  • Non workshop day: Eary arrival and registration 
  • Facility tours
  • Buffet dinner and drinks 

Tuesday 12th September

Tuesday 12th September  
  • Arrival and registration 
  • Lectures on technique theory
  • Lunch 
  • Sample prep (Group1) / SIM (Group 2) / TXM (Group 3)

Wednesday 13th September

Wednesday 13th September 
  • Lectures on best practice 
  • Lunch 
  • Sample prep (Group 2) / SIM (Group 3) / TXM (Group 1)
  • Workshop dinner - location TBC 

Thursday 14th September

Thursday 14th September  
  • Lectures on software and analysis tools for SXT data 
  • Lunch 
  • Sample prep (Group 3) / SIM (Group 1) / TXM (Group 2)

Friday 15th September

Friday 15th September 
  • Non workshop day 
  • B24 team and cabin will be available for consolidation of learning


For any further information about this workshop, please contact the Diamond Events team.

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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