Adam Crawshaw

My research interests include the development of sample preparation methods for microcrystal crystallography for both X-ray diffraction using VMXm and electron diffraction (ED/microED/3DED). This involves the development of new methods for mounting microcrystals to obtain high quality diffraction data with minimal sample usage. I am also interested in the possibilities for the processing of samples between cryo-cooling of the sample and the diffraction experiment using methods such as focused ion beam milling. During the building and commissioning of VMXm, ED of protein crystals has grown significantly. The sample envelope between X-ray and ED has become closer than ever through the development of VMXm. As such, I am particularly interested in the crossover between X-ray diffraction and ED, specifically the sample size requirements, the information content of such experiments and the radiation damage that each type of radiation can generate.

• Keown RJ, Crawshaw AD, Trincao J, Carrique L, Gildea RJ, Horrell S, Warren AJ, Axford D, Owen R, Evans G, Bezier A, Metcalf P and Grimes JM. Atomic structure of a nudivirus occlusion body protein determined from a 70-year-old crystal sample. Nature Communications. (2023)14:4160 DOI:10.1038/s41467-023-39819-1
• Parkhurst JM, Crawshaw AD, Siebert CA, Dumoux M, Owen CD, Nunes P, Waterman D, Glen T, Stuart DI, Naismith JH and Evans G. Investigation of the milling characteristics of different focused-ion-beam sources assessed by three-dimensional electron diffraction from crystal lamellae. IUCrJ. 2023:10(3) DOI:10.1107/S2052252523001902
• Crawshaw AD, Beale EV, Warren AJ, Stallwood A, Duller G, Trincao J and Evans G. A sample preparation pipeline for microcrystals at the VMXm beamline. JoVE 2021 DOI: 10.3791/62306

• Beale EV, Warren AJ, Trincao J, Beilsten-Edmands J, Crawshaw AD, Sutton G, Stuart D and Evans G. Scanning electron microscopy as a method for sample visualization in protein X-ray crystallography. IUCrJ. 2020;7(3) DOI:10.1107/S2052252520003875

• Crawshaw AD, Baslé A and Salgado PS. A practical overview of molecular replacement: Clostridiodes difficile PilA1, a difficult case study. 2020;76:1-11 DOI:10.1107/S2059798320000467

• Storm SLS, Crawshaw AD, Devenish NE, Bolton R, Hall DR, Tews I and Evans G. Measuring energy-dependent photoelectron escape in microcrystals. IUCrJ. 2020;7(1):129-135 DOI:10.1107/S2052252519016178

• Tuck L.R., Altenbach K., Ang T.F., Crawshaw A.D., Campopiano D.J., Clarke D.J. and Marles-Wright J. Insight into Coenzyme A cofactor binding and the mechanisms of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans. Sci Rep. 2016 Feb 22;6:22108. DOI: 10.1038/srep22108
• Serrano M., Crawshaw A.D., Dembek M., Monteiro J.M., Pereira F.C., Pinho M.G., Fairweather N.F., Salgado P.S. and Henriques A.O. The SpoIIQ-SpoIIIAH complex of Clostridium difficile controls forespore engulfment and late stages of gene expression and spore morphogensis. Mol Microbiol. 2016 Apr;100(1):204-28. DOI: 10.1111/mmi.13311
• Warren A.J, Crawshaw A.D., Trincao J., Aller P., Alcock S., Nistea I., Salgado P.S. and Evans G. In vacuo X-ray data collection from graphene-wrapped protein crystals. Acta D Biol Crystallogr. 2015 Oct;71(Pt 10):2079-88. DOI: 10.1107/S1399004715014194
• Crawshaw A.D., Serrano M., Stanley W.A., Henriques A.O. and Salgado P.S.. A mother cell-to-forespore channel: current understanding and future challenges. FEMS Microbiol Lett. 2014 Sep;358(2):129-36 DOI:10.1111/1574-6968.12554