Protein formulation and characterisation

Multi-line HPLC with fluorescence detector, UV detector, auto-sampler and fraction collector.

High quality membrane protein samples are essential for structural biology.    This is coupled to a high-throughput screening platform to rapidly identify the most suitable conditions for membrane protein purification.

Visiting scientists can enter the workflow at any point providing protein constructs are compatible with our screening platform.  There is no requirement for fluorescent tags but this will limit some of the optimisation that can be done during expression screening stage.   The membrane protein expression screening platform allows an initial purification on a small scale while membrane protein purification and formulation allows scientists to screen a wider range of conditions including but not limited to detergents, amphipols, membrane scaffold proteins (MSPs), SMALPS, lipids, pH and salts. Please contact the facility for more information.