High quality membrane protein samples are essential for structural biology. This is coupled to a high-throughput screening platform to rapidly identify the most suitable conditions for membrane protein purification.
Visiting scientists can enter the workflow at any point providing protein constructs are compatible with our screening platform. There is no requirement for fluorescent tags but this will limit some of the optimisation that can be done during expression screening stage. The membrane protein expression screening platform allows an initial purification on a small scale while membrane protein purification and formulation allows scientists to screen a wider range of conditions including but not limited to detergents, amphipols, membrane scaffold proteins (MSPs), SMALPS, lipids, pH and salts. Please contact the facility for more information.
Sample preparation for crystallisation and cryo-EM is key for ensuring success. In the MPL we use a variety of biophysical techniques to assess protein quality. Techniques include size exclusion chromatography coupled to a multi-angle light scatter (SEC-MALS), solid-state infra-red spectroscopy, nano-differential scanning fluorimetry (nano-DSF) and negtive stain EM. Other methods available include Surface Plasmon Resonance (SPR), Isothermal Calorimetry (ITC) and Analytical Ultra-Centrifuge (AUC). These techniques available for visiting scientists interested in membrane protein biology.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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