Whether you have membrane protein crystals or stable membrane protein samples for cryo-EM we can assist visiting scientists to full utilise the varied data collection systems at Diamond Light Source and the RCaH, be it on a beamline or an electron microscope. The close proximity of the MPL to the synchotron and electon bioimaging centre (eBIC) provides a unique advantage in alleviating problems in sample transport and facilitates a quick turn around between design of an experiment and execution.
Membrane protein crystals are often very small, extremely fragile, poorly ordered (high mosaicity) and very sensitive to radiation damage. Collecting data from such crystals is not a straightforward task. The MPL combines high-throughput technologies for protein production and crystallisation with the latest developments in X-ray diffraction data collection systems at Diamond such as VMXi. This allows MPL users to characterise early stage crystal hits and determine their promise for optimisation trials leading to a rapid project progression.
The MPL has access to a Thermofisher Glacios with Falcon 4 detector. The Glacios is primarily used for screening of grids and preliminary data analysis but is capable of collecting sufficient data to solve membrane protein structures. With sufficient preliminary data we can help support users to apply for further microscope time through the eBIC user access program.
Data collection support available at the MPL
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