Sofia Jaho

My main research interests are methods and instrumentation development for macromolecular crystallography. I am a Chemical Engineer and started my research career with the study of crystallisation kinetics and the phase diagram of small molecules and proteins, the first important steps to growing well-diffracting crystals for structure determination using X-rays. I am particularly interested in developing methods and tools, exploiting technologies such as micro- and nanofluidics that can make the whole pipeline from crystal to 3D structure easier from the user’s perspective.

I joined Diamond Light Source and the I24 team in 2022 working on developing methods for serial crystallography at synchrotron (SSX) and XFEL (SFX) sources. I am working on optimising crystallisation and serial data collection strategies for experiments of oxygen and/or light sensitive samples. I’m also collaborating with researchers from Diamond and the University of Essex to investigate the ‘on-demand’ photo-induced initiation of reactions using photocages for time-resolved crystallography at synchrotrons and XFELs. My work also includes advances in complementary methods integrated on I24 to validate crystal structures, focusing on the use of online optical spectroscopy on protein crystals that have a spectroscopic signature

I work in collaboration with groups from the University of Essex and the University of Southampton as part of my current research, as well as, with scientists from other MX beamlines and the XFEL hub at Diamond.

 

A list of my publications can be found below.

1. Use of fixed targets for serial crystallography

S. Jaho, D. Axford, D-H. Gu, M.A. Hough, R.L. Owen

Meth. Enzymol. 2024, https://doi.org/10.1016/bs.mie.2024.10.002

2. Spectroscopically Validated pH-dependent MSOX Movies Provide Detailed Mechanism of Copper Nitrite Reductases

S.L. Rose, F.M. Ferroni, S. Horrell, C. Dante Brondino, R.R. Eady, S. Jaho, M.A. Hough, R.L. Owen, S.V. Antonyuk, S.S. Hasnain

J. Mol. Biol. 2024, 436 (18), 168706, https://doi.org/10.1016/j.jmb.2024.168706

3. Hemoglycin visible fluorescence induced by x rays

M.W. McGeoch, R.L. Owen, S. Jaho, J.E.M. McGeoch

J. Chem. Phys. 2023, 158, 11490, https://doi.org/10.1063/5.0143945

4. Microdialysis on-chip crystallization of soluble and membrane proteins with the MicroCrys platform and in situ X-ray diffraction case studies

S. Jaho, Y. Sallaz-Damaz, M. Budayova-Spano

Cryst. Eng. Comm. 2023, 25, 5513-5523, https://doi.org/10.1039/D3CE00466J

5. Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

S. Jaho, N. Junius, F. Borel, Y. Sallaz-Damaz, J.-B. Salmon, M. Budayova-Spano

JoVE 2021, 170, e61660, https://dx.doi.org/10.3791/61660

6. A microfluidic device for both on-chip dialysis protein crystallization and in situ X-ray diffraction

N. Junius, S. Jaho, Y. Sallaz-Damaz, F. Borel, J.-B. Salmon, M. Budayova-Spano

Lab on Chip 2020, 20 (2), 296–310, https://doi.org/10.1039/C9LC00651F

7. Struvite precipitation and COD reduction in a two-step treatment of olive mill wastewater

A.G. Pantziaros, S. Jaho, I. Karga, I.C. Iakovides, P.G. Koutsoukos, C.A. Paraskeva

J. Chem. Technol. Biotechnol. 2018, 93, 730–735, https://doi.org/10.1002/jctb.5422

8. Precipitation of Calcium Carbonate in Porous Media in the Presence of n-Dodecane

S. Jaho, V. Sygouni, S.G. Rokidi, J. Parthenios, P.G. Koutsoukos, C.A. Paraskeva

Cryst. Growth Des. 2016, 16 (12), 6874–6884, https://doi.org/10.1021/acs.cgd.6b01048

9. Experimental Investigation of Calcium Carbonate Precipitation and Crystal Growth in One- and Two-Dimensional Porous Media

S. Jaho, G.D. Athanasakou, V. Sygouni, M.G. Lioliou, P.G. Koutsoukos, C.A. Paraskeva

Cryst. Growth Des. 2016, 16 (1), 359–370, https://doi.org/10.1021/acs.cgd.5b01321