Alistair Siebert

Email: [email protected]
Tel: +44 (0) 1235 778356

•    Methods development for high-energy electron diffraction instrumentation
•    Structure function relationships in photosynthetic membrane proteins
•    Methods development in and applications of cryoET on complex systems


Alistair Siebert was appointed as the HeXI PBS in 2021. Prior to arriving at Diamond as one of the founding employees of eBIC he was the senior EM scientist for five years at the Division of Structural Biology, Oxford. Postdoctoral research fellowships were performed in cryoET and cryoEM of membrane proteins at the New York Structural Biology Centre and the Biocentrum Basel. His longstanding interests in membrane protein structural biology and cryoEM began as a PhD student between the laboratories of Neil Hunter and Per Bullough during studies of the photosynthetic apparatus of purple bacteria.

Key Research Areas

•    Methods development for high-energy electron diffraction instrumentation
•    Structure function relationships in photosynthetic membrane proteins
•    Methods development in and applications of cryoET on complex systems


•    Pedro Nunes (HeXI)
•    David Farmer (eBIC)

Phd Students:
•    Frederick Hawkins (University of Sheffield with Andrew Hitchcock and Prof. Matthew Johnson)
•    William Bowles (Rosalind Franklin Institute with Prof. Jim Naismith FRS and Maud Dumoux)

Recent Publications

Investigation of the milling characteristics of different focused-ion-beam sources assessed by three-dimensional electron diffraction from crystal lamellae.
Parkhurst JM, Crawshaw AD, Siebert CA, Dumoux M, Owen CD, Nunes P, Waterman D, Glen T, Stuart DI, Naismith JH, Evans G. IUCrJ. 2023 May 1. doi: 10.1107/S2052252523001902.PMID: 36952226

Cryo-EM structure of the four-subunit Rhodobacter sphaeroides cytochrome bc1complex in styrene maleic acid nanodiscs.
Swainsbury DJK, Hawkings FR, Martin EC, Musiał S, Salisbury JH, Jackson PJ, Farmer DA, Johnson MP, Siebert CA, Hitchcock A, Hunter CN. Proc Natl Acad Sci U S A. 2023 Mar 21;120(12):e2217922120. doi: 10.1073/pnas.2217922120. Epub 2023 Mar 13.PMID: 36913593

Cryo-plasma FIB/SEM volume imaging of biological specimens.
Dumoux M, Glen T, Smith JLR, Ho EML, Perdigão LMA, Pennington A, Klumpe S, Yee NBY, Farmer DA, Lai PYA, Bowles W, Kelley R, Plitzko JM, Wu L, Basham M, Clare DK, Siebert CA, Darrow MC, Naismith JH, Grange M. Elife. 2023 Feb 21;12:e83623. doi: 10.7554/eLife.83623.PMID: 36805107 

Application of super-resolution and correlative double sampling in cryo-electron microscopy.
Sheng Y, Harrison PJ, Vogirala V, Yang Z, Strain-Damerell C, Frosio T, Himes BA, Siebert CA, Zhang P, Clare DK. Faraday Discuss. 2022 Nov 8;240(0):261-276. doi: 10.1039/d2fd00049k.PMID: 35938521 

Cryo-EM structures of the Synechocystis sp. PCC 6803 cytochrome b6f complex with and without the regulatory PetP subunit.
Proctor MS, Malone LA, Farmer DA, Swainsbury DJK, Hawkings FR, Pastorelli F, Emrich-Mills TZ, Siebert CA, Hunter CN, Johnson MP, Hitchcock A. Biochem J. 2022 Jul 15;479(13):1487-1503. doi: 10.1042/BCJ20220124.PMID: 35726684 

Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast.
Ma OX, Chong WG, Lee JKE, Cai S, Siebert CA, Howe A, Zhang P, Shi J, Surana U, Gan L. PLoS One. 2022 Apr 14;17(4):e0266035. doi: 10.1371/journal.pone.0266035. eCollection 2022.PMID: 35421110

2.4-Å structure of the double-ring Gemmatimonas phototrophica photosystem.
Qian P, Gardiner AT, Šímová I, Naydenova K, Croll TI, Jackson PJ, Nupur, Kloz M, Čubáková P, Kuzma M, Zeng Y, Castro-Hartmann P, van Knippenberg B, Goldie KN, Kaftan D, Hrouzek P, Hájek J, Agirre J, Siebert CA, Bína D, Sader K, Stahlberg H, Sobotka R, Russo CJ, Polívka T, Hunter CN, Koblížek M. Sci Adv. 2022 Feb 18;8(7):eabk3139. doi: 10.1126/sciadv.abk3139. Epub 2022 Feb 16.PMID: 35171663 

Parakeet: a digital twin software pipeline to assess the impact of experimental parameters on tomographic reconstructions for cryo-electron tomography.
Parkhurst JM, Dumoux M, Basham M, Clare D, Siebert CA, Varslot T, Kirkland A, Naismith JH, Evans G. Open Biol. 2021 Oct;11(10):210160. doi: 10.1098/rsob.210160. Epub 2021 Oct 27.PMID: 34699732

Single Particle Cryo-Electron Microscopy: From Sample to Structure.
White JBR, Maskell DP, Howe A, Harrow M, Clare DK, Siebert CA, Hesketh EL, Thompson RF. J Vis Exp. 2021 May 29;(171). doi: 10.3791/62415.PMID: 34125091

The antigenic anatomy of SARS-CoV-2 receptor binding domain.
Dejnirattisai W, Zhou D, Ginn HM, Duyvesteyn HME, Supasa P, Case JB, Zhao Y, Walter TS, Mentzer AJ, Liu C, Wang B, Paesen GC, Slon-Campos J, López-Camacho C, Kafai NM, Bailey AL, Chen RE, Ying B, Thompson C, Bolton J, Fyfe A, Gupta S, Tan TK, Gilbert-Jaramillo J, James W, Knight M, Carroll MW, Skelly D, Dold C, Peng Y, Levin R, Dong T, Pollard AJ, Knight JC, Klenerman P, Temperton N, Hall DR, Williams MA, Paterson NG, Bertram FKR, Siebert CA, Clare DK, Howe A, Radecke J, Song Y, Townsend AR, Huang KA, Fry EE, Mongkolsapaya J, Diamond MS, Ren J, Stuart DI, Screaton GR. Cell. 2021 Apr 15;184(8):2183-2200.e22. doi: 10.1016/j.cell.2021.02.032. Epub 2021 Feb 18.PMID: 33756110 

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