Electron Cryo Microscopy in Structural Biology

Apr 26. till Apr 29.

Electron Cryo Microscopy in Structural Biology

This course ran in April 2021, please find here a repository of key information and resources from the event.

Date
26/04/20212021-04-26T09:45:00 - 29/04/20212021-04-29T16:00:00
Time
09:45 - 16:00
Location
Diamond Light Source, Oxfordshire, UK

Introduction

To provide fundamental and practical lectures in cryo-EM, from the electron microscope and sample preparation, through to image formation and processing, reconstruction in SPA/cryo-ET and model building.

This course was run entirely remotely and targeted at the Oxford Wellcome Trust Doctoral Training Program (WT DTP) students but open to the wider community via a virtual platform. Remote practical sessions were also ran covering sample preparation, cryo-EM screening, data collection and cryo-ET.

Where possible the slides and recordings of the lecturers are provided for download in the programme below. Note: Videos will be available soon.

Programme

Download programme

Day 1: Monday, April 26th

Lecture 1: Why cryogenic electron microscopy (cryo-EM)? – Kyle Morris (eBIC)

Slides

Lecture 2: The electron microscope – Karen Davies (eBIC)

Slides

Lecture 3: Sample preparation – Dimple Karia (Thermo Fisher Scientific) 

Slides | Lecture video

Practical 1: Cryogenic sample preparation for cryo-EM – Kyle Morris (eBIC)

Practical video

Day 2: Tuesday, April 27th

Lecture 4: Image formation – Dan Clare (eBIC)

Slides | Lecture video

Lecture 5: Image processing – Colin Palmer (CCP-EM)

Slides | Lecture video

Lecture 6: From 2D images to 3D density maps I: SPA – Chris Aylett (Imperial)

Slides | Lecture video

Practical 2: Screening samples in cryo-EM – Peter Harrison (eBIC)

 

Day 3: Wednesday, April 28th

Lecture 7: From 2D images to 3D density maps II: Cryo-ET – Tanmay Bharat (Oxford)

Lecture video

Lecture 8: Averaging and reconstructing 3D maps in cryo-ET – Ben Himes (HHMI/UMassMed)

 

Lecture 9: Fitting and building of atomic models – Agnel-Praveen Joseph (CCP-EM)

 

Practical 3: Single-Particle Averaging data collection demonstration – Vinod Vogirala (eBIC)

 

Day 4: Thursday, April 29th

 

Practical 4: Cryo-ET data collection demonstration - Julika Radecke (eBIC)

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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