This course ran in April 2021, please find here a repository of key information and resources from the event.
To provide fundamental and practical lectures in cryo-EM, from the electron microscope and sample preparation, through to image formation and processing, reconstruction in SPA/cryo-ET and model building.
This course was run entirely remotely and targeted at the Oxford Wellcome Trust Doctoral Training Program (WT DTP) students but open to the wider community via a virtual platform. Remote practical sessions were also ran covering sample preparation, cryo-EM screening, data collection and cryo-ET.
Where possible the slides and recordings of the lecturers are provided for download in the programme below. Note: Videos will be available soon.
Day 1: Monday, April 26th 2021
Lecture 1: Why cryogenic electron microscopy (cryo-EM)? – Kyle Morris (eBIC)
Lecture 2: The electron microscope – Karen Davies (eBIC)
Lecture 3: Sample preparation – Dimple Karia (Thermo Fisher Scientific)
Practical 1: Cryogenic sample preparation for cryo-EM – Kyle Morris (eBIC)
Day 2: Tuesday, April 27th 2021
Lecture 4: Image formation – Dan Clare (eBIC)
Lecture 5: Image processing – Colin Palmer (CCP-EM)
Lecture 6: From 2D images to 3D density maps I: SPA – Chris Aylett (Imperial)
Practical 2: Screening samples in cryo-EM – Peter Harrison (eBIC)
Day 3: Wednesday, April 28th 2021
Lecture 7: From 2D images to 3D density maps II: Cryo-ET – Tanmay Bharat (Oxford)
Lecture 8: Averaging and reconstructing 3D maps in cryo-ET – Ben Himes (HHMI/UMassMed)
Lecture 9: Fitting and building of atomic models – Agnel-Praveen Joseph (CCP-EM)
Practical 3: Single-Particle Averaging data collection demonstration – Vinod Vogirala (eBIC)
Day 4: Thursday, April 29th 2021
Practical 4: Cryo-ET data collection demonstration - Julika Radecke (eBIC)
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