Industrial Liaison Office
Welcome to I04
8th June 2020: Access to MX beamlines is currently operating under new guidelines. Please read carefully.
I04 variable and microfocus MX
I04 is one of seven macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9537 Å (13.00 keV) but it is tuneable over the wavelength range 0.69 - 2.066 Å (6 keV - 18 keV). On request it is also possible to work at a wavelength as low as 0.62 Å (20keV).
The beamsize can be focussed from 10 x 5 µm2 to 110 x 100 µm2 (h x v) across the available energy range. On request smaller beams in the vertical direction are possible. This will primarily depend on the working wavelength.
All MX beamlines use SPINE standard pins and Unipucks from cryocooled samples. The beamline can be accessed remotely.
The beamline is now equipped with the first Eiger2 XE 16M detector which will enable faster data collection and new data collection protocols.
I03 and I04 are equipped with Eiger2 XE 16M detectors - collect at up to 560 Hz!
Smargon and new goniometer positioners installed - fast grid scans available.
New monochromator for I04. Big improvements, more to learn and deliver to users over coming months.
New BART robot installed on I04 - 37 pucks, 592 samples, 2.5x faster exchange times!
Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.
Crystal washer now available
I04 installed a Pilatus 6M-F detector in April 2013.
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Automated Single Crystal Crystallography
Example of using I04 for structural characterization
Current Status
- I04 flux profile for 30 x 20 micron (h x v) beam size
| Item | Available |
|---|---|
| Microfocus Beam | ✓ |
| Variable Focus Beam | ✓ |
| Fluorescence data collection | ✓ |
| Tuneable MAD | ✓ |
| Interleaved energy data collection | |
| Inverse beam data collection | |
| Multi-axis goniometry | ✓ |
| IMISX Capable for LCP in situ studies - discuss with staff before arrival | ✓ |
| In situ goniometry for crystallisation plate | ✗ |
| Automated strategy calculation | ✓ |
| Automated data processing | ✓ |
| Automated difference map calculation | ✓ |
| Automated phasing | ✓ |
| Automated molecular replacement | ✓ |
| Remote Access | ✓ |
I04 Specifications
| Standard energy (Å / keV) | 0.9537 / 13.00 |
| Flux at 13.000 keV (ph/s) | 1.25 x 1012 |
| Wavelength range (Å) | 0.69 - 2.07 |
| Energy range (keV) | 6.0 - 18.0 |
| Beam size at sample (µm) | 10 x 5 --> 110 x 100 (h x v) |
| Detector | Eiger2 XE 16M |
| Time to collect 180o data set (seconds) | 3.2 + |
| Sample Changer | BART |
| No. of pins in dewar | 592 |
| Sample exchange time (seconds) | 18 |
| Guide to samples screened/hour (user centering) | 30 |
+ 0.1o per 0.0018 second exposure 1800 frames (shutterless)
Features include:
- Variable focus beam from 10 x 5 to 110 x 100 µm delivering 6.7 x 1011 ph/s at the Se K edge and 1.25 x 1012 ph/s at 13.0 keV (20 micron beam size at 300 mA ring current)
- Dectris Eiger2 XE 16M detector
- Smargon multi-axis goniometer
- High resolution viewing system
- Accurate sample centering stages
- Simple beam visualisation procedures
- Fine resolution attenuation control
- DLS BART sample changer - 37 Unipucks / 592 samples
- Vortex fluorescence detector
- Automated data processing
- Remote access
