Small angle X-ray scattering (SAXS) from biological macromolecules such as proteins (also called bioSAXS) gives information on the size and shape of macromolecules in solution. It is increasingly used by the pharmaceutical industry to provide details of the radius of gyration and molecular weight of a protein complex (e.g. multi sub-unit complexes), significant conformational changes upon ligand binding, screening of structural changes with formulation conditions (e.g. pH, ionic strength, additives, temperature etc) and many more applications.
With high throughput and fast and reliable measurements, bioSAXS at Diamond enables low volume samples to be studied at close to native state conditions. It is a very effective tool in situations where crystals are unattainable or where samples may be challenging eg. flexible proteins, or multi-sub unit complexes (e.g antibody binding studies). The highest quality data can be achieved using online size exclusion chromatography (SEC-SAXS), ideal for advanced modelling. Online MALS is also available, allowing validation of laboratory based studies.
Often used in conjunction with structural information from crystallography or electron microscopy bioSAXS may form part of a wider set of research, made simple by the co-location of Diamond’s broad portfolio of synchrotron and cryoEM techniques, all housed on one central site.
Our expert bioSAXS team can support and advise you in how to get the best from your research using this technique and are on hand to support you throughout your experiment.