Instruments by Science Group
Macromolecular
Soft Condensed 
Imaging and
Biological
Magnetic
Structures and
Crystallography 
Spectroscopy XFEL-Hub contact
XFEL-Hub Principal Scientist:
Allen Orville
Tel: +44 (0) 1235 567505
E-mail: [email protected]
Fixed target sample delivery
Fixed target sample delivery is an efficent method to collect serial crrystallography at Synchrotrons and XFELs. I24 developed a fast xyz stage to collect serial data. If you are interested in using fixed target method during your own BAG beamtime, please check I24 SSX webpages.
Fixed Target data collection: from crystals to structure
Time-resolved experiment, Drop-on-Chip (DoC)
Fixed target sample delivery can enable time-resolved mixing experiment as shown previously by Mehrabi et al., 2019 with the HARE setup.
We developed a similar system using the same piezoelectric injector (autodrop pipette from microdrop technologies) to eject 50-70 pL droplet of ligand/substrate onto micro-crystals sat in the chip aperture (see our pre-print).
We can probe mixing time ranging from 5ms to 10 seconds. If you are interested, please contact us for more details
Substrate delivered every other wells with interleaved control.
References:
Mehrabi P, Schulz EC, Agthe M, Horrell S, Bourenkov G, von Stetten D, Leimkohl JP, Schikora H, Schneider TR, Pearson AR, Tellkamp F, Miller RJD. Nat Methods. 2019 Oct;16(10):979-982.
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Oxfordshire
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