Serial Synchrotron Crystallography (SSX) is an emerging method driven, in part, by the sample requirements imposed by X-ray Free Electron Lasers. Serial techniques can also be exploited at synchrotrons, especially at microfocus beamlines, opening up many new opportunities. At I24 we are developing serial approaches and a number of these are now ready for use. Our expertise is in fixed target serial crystallography but other approaches are also available (see below).
Fixed Target Serial Synchrotron Crystallography (FT-SSX) is attractive as it potentially offers high hit-rates coupled with modest sample consumption. Further, the same approach can be used almost without modification at both synchrotrons and FELs.
Our fixed target approach makes use of silcon nitride 'chips' has been developed over the last few years in collaboration with the Miller groups in Toronto and Hamburg. We have recently developed our own chips and have collected good quality data using them at both I24 and XFEL sources. The current generation of chip is capable of holding >25,000 crystals.
Chips are mounted on a high-speed, high-precision xyz stage mounted at the sample position. This setup allows data collection from all 25,600 positions on a chip in less than 10 minutes.
A LCP extruder can be mounted at I24 relatively easily with data collection available through a staff written gui. We are working on improving the implementation of this and also implementation at other beamlines. If you're interested please get in touch.
Thin films can provide an extremely convenient means of high throughput room temperature data collection especially when the number of crystals available is limited.
Fixed Target data collection: from crystals to structure
High viscosity extruder: from crystals to structure
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
Copyright © 2020 Diamond Light Source
Diamond Light Source Ltd
Harwell Science & Innovation Campus