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Soft Condensed Matter - Small Angle Scattering
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B21 Contact

Beamline phone numbers:
+44 (0) 1235 77 8627
+44 (0) 1235 77 8640

Principal Beamline Scientist: 
Nathan Cowieson

Tel: +44 (0) 1235 567 504
E-mail: nathan.cowieson@diamond.ac.uk

More

B21 High Throughput SAXS

Status: Operational

Beamsize: <75 μm
Detector: Eiger 4M
Energy: 6 - 23 keV

SAXS: Small Angle X-ray Scattering Humidity Control Remote Access Scattering
  1. Instruments
  2. Soft Condensed Matter
  3. Small Angle Scattering
  4. B21

Welcome to B21

B21: Solution State SAXS

  • Apply for Rapid Access
  • Apply for Beamtime
  • Sample shipment & shipping form
  • Learn About BioSAXS
  • Upload publication(s)

 

  • Rg-based dimensionless Kratky plot of SEC purified BSA (red) versus unpurified (black)
  • SAXS-directed MD modelling of an RNA
1 2

B21 is a dedicated solution state Small Angle X-ray Scattering (SAXS) beam line that can accommodate a wide variety of aqueous-based and some limited organic solvent-based samples. B21 utilises a bending magnet source and delivers ~3 x 10^12 photons/sec directly to the sample.

The photons are distributed over a large 0.8 x 2 mm cross-section which is particularly helpful in minimising radiation damage while enhancing the signal of the particles. Our detector is currently configured to measure a scattering vector (q) range from 0.0032 to 0.38 Å-1.

 SAXS time is offered through an application process via our users office. 

Our end-station can be configured for full-automation from a 96-well plate, using the BIOSAXS robot (designed by the EMBL in Grenoble). However, we do offer Size-Exclusion Chromatography (SEC) coupled SAXS with either Superdex or Shodex SEC columns controlled by an Agilent HPLC. SAXS samples are measured through a temperature controlled capillary.

Shipping Samples

SHIPPING

Samples should be shipped with the following information. A sheet of paper with a sample list (see shipping form above) and please include the following:

Name of Local Contact

Diamond Visit ID

Date of Visit

Name of Experimenter

Name of Home Institution

 

We highly recommend for samples on wet-ice that all samples be placed in a zip-lock bag.  For samples on dry ice, the bag should be wrapped in paper-towels and taped.

 

Please address samples to:

Name of Local Contact and B21

Diamond Light Source Limited, Chilton, Didcot, Oxfordshire, OX11 0DE

 

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