cryo-EM services

Stages of a typical cryo-EM experiment

Description	Cryo-EM screening (2 days) to evaluate the sample’s suitability for cryo-EM
Activity	Cryo-EM grid preparation (this can either be done at Diamond or at your own laboratory) Cryo-EM grid screening 2D classification (as appropriate) Learn the cryo-EM basics by shadowing our specialists and participating in the experiments
Deliverables	Sample is suitable to ‘Explore’

Description	Higher throughput data collection to determine near atomic resolution insights into binding modes, enable drug-design and/or validate modes-of-action
Activity	Optimisation of grid preparation Cryo-EM grid screening on the Glacios Collection of high resolution data sets on the Krios 3D reconstruction (optional) Building and refinement of atomic models (optional) Learn cryo-EM theory
Deliverables	3D reconstruction Initial molecular model

Description	Full cryo-EM tool access via an open membership model Shared learning
Activity	Applications expert guidance and support Practical training programme
Deliverables	Become confident in routinely solving the most difficult structures Engage with the cryo-EM community by attending one of our regular events