Cryo-ET for Beginners: From sample to structure

Nov 03. till Nov 06.

Cryo-ET for Beginners: From sample to structure

This virtual beginners’ course is designed for PhD students and early career researchers who are new to cryo-electron tomography (cryo-ET) and want to incorporate it into their research.

Date
03/11/20252025-11-03T09:00:00 - 06/11/20252025-11-06T17:00:00
Time
09:00 - 17:00
Location
Online
Link
Register here

For more information or questions about this event please contact [email protected]

This online virtual beginners’ course is designed for PhD students and early career researchers who are new to cryo-electron tomography (cryo-ET) and want to incorporate it into their research. The programme combines core lectures with hands-on and live demonstrations sessions guiding participants through the full cryo-ET workflow:

  • sample preparation and vitrification techniques
  • cryo-CLEM and CEMOVIS approaches
  • lamella lift-out preparation with FIB-SEM
  • tomography data collection on Titan Krios microscopes
  • introduction to data processing, from segmentation to sub-tomogram averaging in RELION 5

The course offers a complete foundation in cryo-ET, from sample to structure, in an accessible virtual format.

Register here

The registration deadline is Wednesday 8 October 2025.

Eligibility

Applicants must meet the following criteria:

  • be a PhD student or postdoc new to the field and will be using cryo-EM as part of your work
  • have access to the necessary cryo-EM instrumentation at your home institution or through a local facility or have established plans to secure such access in the near future

Programme Schedule

The course begins with introductory lectures on in situ structural biology, vitrification techniques, cryo-ET principles, covering CEMOVIS and cryo-CLEM. Participants will be asked to give a short flash-talks to present themselves and their research. The following days focus on practical demonstrations of lamella preparation lift-out using Hydra pFIB-SEM, alongside hands-on Krios sessions for grid screening and tomography data collection. The final day is dedicated to cryo-ET data processing, with lectures on segmentation and sub-tomogram averaging and a live demonstration in RELION.

Organisers

Astbury Biostructure Laboratory, University of Leeds

eBIC, Diamond Light Source

Midlands Regional Cryo-EM Facility, University of Leicester

Birkbeck college, University of London

Scottish Centre for Macromolecular Imaging, University of Glasgow

Funding Acknowledgments

If your research or publications have benefitted from attending this training event, we kindly ask that you acknowledge this support by citing the Wellcome/MRC cryo‑EM training programme (grant number 218785/Z/19/Z) in any resulting publications.

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

Diamond Light Source Ltd
Diamond House
Harwell Science & Innovation Campus
Didcot
Oxfordshire
OX11 0DE

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