Wellcome Trust/MRC- Beginners Single Particle Cryo-EM course 2024

Sep 23. till Sep 27.

Wellcome Trust/MRC- Beginners Single Particle Cryo-EM course 2024

The Astbury Biostructure Lab (University of Leeds) is partnering with eBIC (Diamond light source) Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Facility (University of Leicester) and Birkbeck college (University of London) to host training courses between 2021-2026 in cryo-EM. This training program would not be possible without these partners, and is funded by Wellcome and MRC (218785/Z/19/Z).

Date
23/09/20242024-09-23 - 27/09/20242024-09-27
Location
The Astbury Biostructure Laboratory (University of Leeds)
Link
Register here

Overview

This course is a part of our cryo-EM Wellcome/MRC training program tailored for PhD students and postdocs who wish to use single particle cryo-EM for their research but have very little or no prior experience in this method.

The aim of the course is to give a theoretical knowledge and practical experience in the entire cryo-EM single particle analysis workflow including negative stain TEM, cryo-EM sample preparation, cryo-EM screening and data collection using our Titan Krios microscopes, and an introductory overview of the data processing.

The course is limited to 12 participants, which are encouraged to send their protein samples or cryo-EM grids for testing, screening and potential data collection during the practical sessions. Samples should be delivered no later than September 6th.

We offer financial support for travel and accommodation to participants with limited access to funding. This support includes reimbursement of travel expenses up to £250, and accommodation for five nights in a budget hotel. Any expenses exceeding these amounts will need to be covered by the participants.

Programme

Day 1 will begin with lectures and theory. In the afternoon, participants will present their research projects during a poster session.

Days 2-4: Attendees will engage in practical sessions organized in small groups of three or four. These sessions will cover a variety of topics, including:

  • Negative stain grid preparation and screening
  • Cryo-EM grid preparation
  • Cryo-grid screening on a Titan Krios microscope
  • Setting up a data collection

Each participant will receive approximately 3 hours of direct, hands-on experience with Titon Krios microscope. Additionally, they will have the opportunity to observe and learn from their peers.

Day 5 will be dedicated to image processing concepts and key software. Participants will also engage in hands-on processing of a small model dataset.

For the image processing each participant will be required to bring their own laptop and a mouse.

Contact

The course is organised by The Astbury Biostructure Laboratory (University of Leeds) together with Midlands Regional Cryo-EM Facility (University of Leicester) in partnership with Birkbeck college (University of London), Scottish Centre for Macromolecular Imaging (University of Glasgow) and eBIC (Diamond light source).

Please contact [email protected] for more information.

Funding Acknowledgments

If your research or publications have benefitted from attending this training event, we kindly ask that you acknowledge this support by citing the Wellcome/MRC cryo‑EM training programme (grant number 218785/Z/19/Z) in any resulting publications.

Diamond Light Source

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