Cryogenic Electron Microscopy in Structural Biology

Aug 14. till Aug 17.

Cryogenic Electron Microscopy in Structural Biology

This course will provide fundamental lectures and practical demonstrations in cryo-EM, from sample preparation and the electron microscope, through to image formation and processing, reconstruction in SPA and cryo-ET, and model building.

Date
14/08/20232023-08-14 - 17/08/20232023-08-17
Location
Diamond Light Source and online via zoom

Overview

This course will provide fundamental lectures and practical demonstrations in cryo-EM, from sample preparation and the electron microscope, through to image formation and processing, reconstruction in SPA and cryo-ET, and model building. This is a hybrid event targeted at researchers new to the field, lectures will be broadcast to the wider community via Zoom and a limited number of in-person places are available for Oxford Wellcome Trust DTP students and Harwell Campus students & staff. Practical sessions covering sample preparation, cryo-EM screening, SPA data collection and cryo-ET are in-person and strictly limited to first year Oxford Wellcome Trust DTP students.

Organisers: Lorna Malone (eBIC), Kyle Morris (eBIC), Tom Burnley (CCPEM) & Emma Phillips (CCPEM)

Agenda

Download programme here

Monday 14th August 2023

09:30–09:45

Arrival at RAL reception (R75) for students and external visitors

09:45–10.00

Introductions and welcome in G59 – Dan Clare (Diamond Light Source)

10:00–10:30

Lecture 1: Why cryogenic electron microscopy (cryo-EM)? – Kyle Morris (eBIC)

10:30–11:15

Lecture 2: The electron microscope – Karen Davies (eBIC)

11:15–11:30

Coffee break (G59)

11:30–12:15

Lecture 3: Sample preparation for cryo-EM – Dimple Karia (Thermo Fisher Scientific)

12:15–13.15

Lunch break (R22)

13.15–13:30

Health and safety talk – Karen Davies (eBIC) (G59)

13:30–16:30

Practical 1: Cryogenic sample preparation for cryo-EM – Peter Harrison (eBIC)

 

Tuesday 15th August 2023

09:30–10:15

Lecture 4: Image formation in the electron microscope – Dan Clare (eBIC)

10:15–11:00

Lecture 5: Image processing theoretical introduction – Matt Iadanza (CCP-EM)

11:00–11:15

Coffee break (G59)

11:15–12:00

Lecture 6: From 2D images to 3D density maps in SPA – Yuriy Chaban (eBIC)

12.00–12.30

Lecture 7: SPA processing pipelines – Daniel Hatton (DLS/eBIC)

12:30–13:30

Lunch break (R22)

13:30–16:30

Practical 2: Screening samples in cryo-EM – Peter Harrison (eBIC)

 

Wednesday 16th August 2023

09:30–10:15

Lecture 8: From 2D images to 3D density maps in cryo-ET – Kyprianos Hadjidemetriou,

10:15–11:00

Lecture 9: Averaging and reconstructing 3D maps in cryo-ET – Pranav Shah (Oxford)

11:00–11:15

Coffee break (G59)

11:15–12:00

Lecture 10: Fitting and building of atomic models – Agnel-Praveen Joseph (CCP-EM)

12.00–12.30

Lecture 11: Cryo-ET processing pipelines – Daniel Hatton (DLS/eBIC)

12:30–13:30

Lunch break (R22)

13:30–16:30

Practical 3: Single-Particle Averaging data collection demonstration – Vinod Vogirala (eBIC)

 

Thursday 17th August 2023

09:30–12:30

Practical 4: Cryo-ET data collection demonstration – Davide Zabeo (eBIC)

12:30–13:30

Lunch break (R22)

13:30–14:45

Research talks by eBIC PDRA & PhD students (I14 1-10)

14:45–15.00

Coffee break (I14 1-10)

15.00–16.00

DTP student presentations: Student learning & feedback talks (I14 1-10)

16.00–16:30

Wrap-up Q&A

 

Access to the event

There is no fee to attend this course, but registration is mandatory.

The event will include lectures and practical demonstrations.

  • Lectures are open to everyone and will be delivered both in-person and online.
  • Practical demonstrations are reserved only to 1st Year Oxford Wellcome Trust DTP students.

Please note that due to limited space, in-person tickets are reserved for 1st Year Oxford Wellcome DTP Students and Harwell Campus attendees only.

Registration

Registration for the event is now open and will close on 7th August 2023 10:00 AM (UK time).

Register here

Contact the organisers

If you have any queries relating to this workshop, please contact the Diamond Events Team who will be able to help.

Training recordings from August 2023 Workshop

Please find below recordings of the talks delivered at our 2023 Cryogenic Electron Microscopy in Structural Biology Workshop. 

Please note the following recordings are unfortunately not available; 

  • Lecture 2 - slides available to view here.
  • Lecture 5 - slides available to view here. 
  • Lecture 9 and Practicals 3 & 4 (no resources available). 

Lecture 1: Why cryogenic electron microscopy (cryo-EM)? – Kyle Morris (eBIC)

Lecture 3: Sample preparation for cryo-EM – Dimple Karia (Thermo Fisher Scientific)

Lecture 4: Image formation in the electron microscope – Dan Clare (eBIC)

Lecture 6: From 2D images to 3D density maps in SPA – Yuriy Chaban (eBIC)

Lecture 7: SPA processing pipelines – Daniel Hatton (DLS/eBIC)

Lecture 8: From 2D images to 3D density maps in cryo-ET – Kyprianos Hadjidemetriou

Lecture 10: Fitting and building of atomic models – Agnel-Praveen Joseph (CCP-EM)

Lecture 11: Cryo-ET processing pipelines – Daniel Hatton (DLS/eBIC)

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