Wellcome-iNEXT Cryogenic Electron Microscopy in Structural Biology 2024

May 28. till May 31.

Wellcome-iNEXT Cryogenic Electron Microscopy in Structural Biology 2024

This course will provide fundamental lectures and practical demonstrations in cryo-EM, from sample preparation and the electron microscope, through to image formation and processing, reconstruction in SPA and cryo-ET, and model building.

This is a hybrid event targeted primarily at PhD students from our internal PhD programme and selected non-UK based PhD students from the community. Lectures will be made open to the wider community via Zoom. Demonstrations and practical sessions covering sample preparation, cryo-EM screening, SPA data collection and cryo-ET are limited to in-person attendees only.

Date
28/05/20242024-05-28T09:00:00 - 31/05/20242024-05-31T17:00:00
Time
09:00 - 17:00
Location
In-person, Diamond Light Source (and online via zoom)
Link
Apply (in-person) or register (virtual) here

Overview

This is a hybrid event targeted at researchers new to the field, it will provide fundamental lectures and practical demonstrations in cryo-EM, from sample preparation and the electron microscope, through to image formation and processing, reconstruction in SPA and cryo-ET, and model building. 

Lectures will be held in-person at Diamond Light Source and will be broadcast to the wider community via Zoom. Demonstrations and hands-on practical sessions covering sample preparation, cryo-EM screening, SPA data collection and cryo-ET will be available to in-person attendees only.

Due to limited space, in-person places are strictly limited to 12 delegates:

  • 7 spaces are reserved for our internal DTP Students (attendance by invitation) and are funded by the Wellcome Trust
  • 5 spaces are available by application to selected PhD students from the community (application details below) - these spaces are available for non-UK based PhD students and are fully funded by iNEXT-Discovery

There is no fee to attend this course in-person or online but registration is mandatory. For the 5 iNEXT funded places, accommodation and food will be provided, however you will need to arrange and pay for your own travel to get to Diamond.

Programme

Full programme coming soon

Day 1: Tuesday 28th May 2024

  • Lectures covering principles of cryo-electron microscopy (EM) and an introduction to Single Particle Analysis, SPA sample preparation and screening strategies (in-person and virtual)
  • Demonstrations in SPA sample preparation, screening and data collection (in-person only)

Day 2: Wednesday 29th May 2024

  • Practical sessions in SPA sample preparation, screening and data collection (in-person only)
  • Lectures covering SPA image processing, reconstruction, dealing with structural heterogeneity in datasets and model building (in-person and virtual)

Day 3: Thursday 30th May 2024

  • Lectures covering principles of cryo-Electron Tomography (ET), sample preparation and screening strategies (in-person and virtual)
  • Demonstrations in cryo-ET sample preparation and data collection (in-person only)

Day 4: Friday 31st May 2024

  • Practical sessions in cryo-ET data collection (in-person only)
  • Lectures covering cryo-ET image processing and reconstruction (in-person and virtual)
  • Student learning & feedback discussion (in-person only)

 

How to Apply

There are 5 iNEXT-Discovery funded spaces available to selected non-UK based PhD students from the community. Places will be allocated on the grounds of merit and anticipated benefit.

All applicants must meet the following criteria:

  • You are a non-UK based PhD student new to the field and will be using cryo-EM as part of your work

Before beginning the application process please prepare the following documents to upload to the application page:

  • A cover letter describing your motivation for applying for the course, and how this will benefit your PhD research.  Please also describe your current role/position and your plans for using EM.
  • A letter of support from your PhD supervisor

If successful, you will be asked to formally accept your place and register for the event. Academic places will be free of charge and includes:

  • all meals and refreshments
  • accommodation if required, checking in on Monday 27th May (bank holiday) and checking out on Friday 31st May.

You will need to arrange and pay for your own travel to get to Diamond.

Key dates

Applications for in-person attendance (for non-UK PhD students): 26th April - 12th May 2024

Candidate selection: All applicants will be notified with the outcome of their application by the 14th May 2024

Registration for online attendance: open from the 26th April - 27th May 2024

 

Contact the organisers

If you have any queries relating to this workshop, or if you are interested in sponsoring this event, please contact [email protected]

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

Copyright © 2022 Diamond Light Source

 

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