Cryo-EM Sample Preparation Workshop

Oct 02. till Oct 04.

Cryo-EM Sample Preparation Workshop

The goal of this workshop is to teach new CryoEM users how to prepare samples for imaging on the cryo electron microscope, primarilary through hands-on tutorials,discussions and introductory talks.

Date
02/10/20172017-10-02T09:00:00 - 04/10/20172017-10-04T17:00:00
Time
09:00 - 17:00
Location
Diamond Light Source


 

Overview

The goal of the workshop is to teach users the best practices in sample preparation for cryoEM imaging. The workshop will be principally hands-on with students preparing vitrified samples on the latest generation of plunge-freezing devices.  They will then have the opportunity to subsequently screen these samples with the eBIC Titan Krios and Talos Arctica electron microscopes.

This workshop will be geared toward novices with the only requirement being that the student, and/or postdoc, shows they plan to use cryoEM as part of their current research. The workshop aims to equip participants with the necessary skill set to further develop their cryoEM expertise at their home laboratories. Several biological test samples will be provided allowing participants hands on experience with a variety of samples.
 

Topics

Introductory Lectures:

  • An introduction to the basics of cryoEM and cryoET
  • The complete guide to sample vitrification
  • Lunch-time bites from sponsors 

Hands-on sessions:

  • Sample preparation for single particle cryoEM
  • Sample screening at eBIC and an introduction to data collection on Titan Krios and Talos Arctica

 

Invited speakers

  • Felix DeHaas, FEI, Eindhoven
  • Chris Russo, LMB Cambridge
  • Rebecca Thompson, University of Leeds
  • Ana Casañal, LMB Cambridge
  • Andrea Nans, The Francis Crick Institute, London
  • Cornelia Cazey, University of Oxford
  • Jan de Weert, Leica Europe
  • Natasha Lukoyanova, Birkbeck, London

 

Local Tutors

  • Yuriy Chaban
  • Dan Clare
  • Michele Darrow
  • Kyle Dent
  • Corey Heckel
  • Alistair Siebert
  • Jason van Rooyen
  • Peijun Zhang

 

How to apply

Applications are now closed.

The successful applicants will be notifed from 14th August 2017.

 

ENQUIRIES

events@diamond.ac.uk

 

 

Venue

The course is residential, with accommodation provided locally. 

Lectures and tutorials will take place at Diamond.

Supported by

       

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

Copyright © 2020 Diamond Light Source

 

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