Cryo-EM Sample Preparation Workshop

Jan 09. till Jan 11.

Cryo-EM Sample Preparation Workshop

The goal of this workshop is to teach users how to prepare samples for imaging on the cryo electron microscope, via a programme of talks, discussions and hands on experience.

Date
09/01/20172017-01-09T09:00:00 - 11/01/20172017-01-11T17:00:00
Time
09:00 - 17:00
Location
Diamond Light Source


 

Overview

The goal of the workshop is to teach users the best practices in sample preparation for cryoEM imaging. The workshop will be composed of lectures, covering the theory behind sample preparation for cryo-EM, as well as hands-on training sessions in the vitrification of selected biological samples. Participants will prepare vitrified samples on the latest generation of plunge-freezing devices and subsequently screen these samples with the eBIC Titan Krios and Talos Arctica electron microscopes.

This workshop will be geared toward novices and aims to equip them with the necessary skill set to further develop their cryo-EM expertise at their home laboratories. Several biological test samples will be proved allowing participants hands on experience with a variety of samples.
 

Topics

Lecture topics:

  • An introduction to cryo-EM and cryo-Tomography
  • An overview of sample preparation 
  • Advanced techniques and new approaches in vitrification

Hands-on sessions:

  • Sample preparation for single particle cryo-EM
  • Sample screening at eBIC and an introduction to data collection on Titan Krios and Talos Arctica

 

Invited speakers

  • Bettina Böttcher, University of Wuerzburg, Germany
  • Sarah Butcher, University of Helsinki, Finland
  • Venkat Dandey, New York Structural Biology Center, US
  • Felix DeHaas, FEI, Eindhoven
  • Christoph Diebolder, NeCEN, Leiden University, Netherlands
  • Kay Grünewald from STRUBI, Oxford University, UK
  • Deryck Mills, Max-Planck Institute, Frankfurt, Germany
  • Peter Peters, Maastricht University, Netherlands
  • Chris Russo, MRC Laboratory, UK
  • Daniel Nemecek, FEI, Eindhoven

 

How to apply

The workshop is free to attend, but numbers are strictly limited to 12.  Places will be allocated on the grounds of merit and anticipated benefit. 

Before beginning the application process please prepare your CV/resume including a description of your experience, other skills, and why you want to attend the course. You will also be required to submit a letter of support from your PhD supervisor (or lab PI if you are a PostDoc). 

KEY DATES 

Applications close -   25th November 2016
Successful applicants notified – 1st December 

ENQUIRIES

events@diamond.ac.uk 

Venue

The course is residential, with accommodation provided at Ridgeway House at Diamond.

Lectures and tutorials will take place at Diamond.

Supported by

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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