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Soft Condensed Matter
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Macromolecular
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B23 Contact

Beamline Phone Number:
+44 (0) 1235 778889

Principal Beamline Scientist:
Giuliano Siligardi

Tel: +44 (0) 1235 778425
E-mail: giuliano.siligardi@diamond.ac.uk

More

B23 Circular Dichroism

Status: Operational

Beamsize: 0.5mm² to 2mm x 4mm
Detector: PMT
Wavelength: 125 - 650 nm
Energy: 2 - 10 eV

Circular Dichroism Spectroscopy In situ Humidity Control Imaging
  1. Instruments
  2. Soft Condensed Matter
  3. B23

Welcome to B23

B23: Circular Dichroism

Circular Dichroism (CD) is the spectroscopic technique to study in solution a wide variety of chiral materials such as small molecules (drugs), polymers and biopolymers (nucleic acids, proteins, carbohydrates and lipids). In particular for proteins, knowledge of the structure-function relationship is essential to dissect the mode of action and to identify new targets for novel drug therapeutics.

For rigid and well structured systems, like enzymes and globular proteins, CD is a low-resolution technique compared to NMR and X-ray crystallography. However, a third to half of mammalian proteins have natively disordered structures that are unsuitable for NMR and X-ray crystallography. CD is the ideal technique to investigate protein/ligand binding interactions of these important systems involved in signal transduction of normal and tumour cells.

B23 produces a collimated beam of small cross section at the sample (about 1mm (V) x 2mm (H)) enabling the measurement of smaller volumes of sample solutions several times better than commercial CD instruments. High photon flux across the UV region improves the signal-to-noise of CD measurements.

B23 is used by researchers in the biological, biochemical, chemical, pharmaceutical, and crystallographic sciences to examine proteins, nucleic acids, carbohydrates, biopolymers, small ligands and the interactions of these molecules to form macromolecular and drug complexes.

CD Imaging molecules. Zinna et al 2017
CD Imaging molecules. Zinna et al 2017
CD of Hydrogels. Sitsanidis Chirality 2018
CD of Hydrogels. Sitsanidis Chirality 2018
Photo UV protein denaturation assay molecules. Hussain et al 2018
Photo UV protein denaturation assay molecules. Hussain et al 2018
Stoichiometry interaction of protein ternary complex frontiers. Siligardi et al 2018
Stoichiometry interaction of protein ternary complex frontiers. Siligardi et al 2018
Tim3 Galactin 9 complex. Silva eBioMedicine 2017
Tim3 Galactin 9 complex. Silva eBioMedicine 2017
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