iNEXT-Discovery Sample Preparation Course

Jan 09. till Jan 12.

iNEXT-Discovery Sample Preparation Course (2023)

Save the date for the upcoming iNEXT-Discovery Sample Preparation Course.

09/01/20242024-01-09T09:00:00 - 12/01/20242024-01-12T16:30:00
09:00 - 16:30
Diamond Light Source, Oxfordshire, UK
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The goal of this iNEXT-Discovery course is to teach users the best practices in sample preparation for single particle analysis cryo-EM imaging. The course will include lectures but otherwise principally be hands-on, with students preparing vitrified samples on  plunge-freezing devices including a demo of the chameleon next generation freezing device. Clipping and loading for Thermo Fisher Scientific autoloaders will also be demonstrated. Students will screen the samples they freeze with an eBIC Titan Krios microscope.

This course will be geared toward researchers who are actively using cryoEM for their research bt are relatively new to the field of cryo-EM. The course is open to academic and industrial applicants. The course is funded by iNext & Diamond Light Source. This funding includes the cost of the course, accommodation and subsidence.  However, you will need to cover the cost of your travel to and from the course. Industrial applicants will be able to access the course on a chargeable basis.

Applicants must show they are using cryo-EM as part of their research and have access to a plunge-freezing device. The course aims to equip participants with the necessary skill set to further develop their cryo-EM expertise at their home institutes and eBIC. Several biological test samples will be provided allowing participants hands on experience with a variety of samples.

Application process for academic and industry participants

Places are strictly limited to 12 academic delegates. Places will be allocated on the grounds of merit and anticipated benefit. 4 industry delegates will be selected by eBIC for industry.

All applicants must meet the following criteria:

• You are using single particle analysis cryo-EM as part of your current research and already have some experience.
• You have access to a plunge freezing device.

Before beginning the application process please prepare the following documents to upload to the registration page:

• A cover letter describing your motivation for applying for the course, and how this will benefit your research and/or facility.  Please also describe your current role/position and your groups plans for using EM.
• A letter of support from your PhD supervisor or lab PI if you are a PostDoc.

Course dates: 16th to 19th October 2023 (Draft agenda below)

June 2023 - Applications open 
31st July 2023 17:00 BST - Applications closes
August 2023 - Application review
Early September 2023 - Selection announcement

All applicants will be notified with the outcome of their application early September 2023.

If successful, you will be asked to formally accept your place by registering online. Academic places will be free of charge and industrial places incur a fee of £4,000 plus VAT (which will be invoiced ahead of the event), both include:

• all meals and refreshments
• accommodation at Ridgeway House based on Harwell Campus, checking in on Sunday 15th October and checking out on Thursday 19th October

You will need to arrange and pay for your own travel to get to Diamond.

Monday 16th October

Monday 16th October 
  • Welcome and orientation 
  • Theoretical and background lectures

Tuesday 17th October

Tuesday 17th October 
  • Plunge freezing and screening 
  • Clipping and loading 

Wednesday 18th October

Wednesday 18th October 
  • Multi-grid collection
  • Chameleon plunge freezing demo

Thursday 19th October

Thursday 19th October 
  • Results review 
  • End of course summary


For any further information about this workshop, please contact the Diamond Events team. For scientific enquiries regarding this workshop, please contact [email protected] or [email protected]

Diamond Light Source

Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.

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