The first automated data set of a test specimen, GroEL, has been successfully collected on Diamond's cryo-electron microscope, Titan Krios I.
The data set was collected with the world's first EPU embedded bioquantum-K2 detector, and was produced in the form of films so that researchers could see a moving image of the specimen. The films were collected in super-resolution counting mode with a 30eV energy selecting slit.
The films were then aligned using IMOD followed by particle selection, alignment and 3D reconstriuction in relion. From a total of 30,000 particles, 17,500 were used to calculate a 3.2Å 3D reconstruction of GroEL. This is currently the highest resolution 3D reconstruction of GroEL determined by cryo-electron microscopy - a significant achievement for Diamond's Titan Krios I.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
Copyright © 2020 Diamond Light Source
Diamond Light Source Ltd
Harwell Science & Innovation Campus
Diamond Light Source® and the Diamond logo are registered trademarks of Diamond Light Source Ltd
Registered in England and Wales at Diamond House, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, United Kingdom. Company number: 4375679. VAT number: 287 461 957. Economic Operators Registration and Identification (EORI) number: GB287461957003.