Kamel El Omari

profilephoto

Kamel is a beamline scientist on I23.

Email: kamel.el-omari@diamond.ac.uk
Tel: +44 (0) 1235 567518

Key Research Area

  • Structural Biology
  • Experimental/native phasing
  • Viral proteins
  1. Research Expertise
  2. Publications
Research Expertise -

Current Research Interests

Viral life cycle
Viral life cycle

Structural biology of viral membrane fusion

The aim of my project is to shed light on proteins interactions occurring between viruses and their host cells. Viruses use host cell resources to carry through their life cycle, but infection triggers host cell antiviral defense mechanisms that in turn drive viral adaptation. Thus it is crucial to understand not only the biology of the virus but also the biology of the host and the different cell pathways hijacked by the virus. This project will initially focus on unravelling the mechanism of viral membrane fusion.

Structural biology of TIP30 complexes

The lack of effective therapies against many cancers and against several viral infections have highlighted the need to firstly understand the mechanisms involved in tumourgenesis and viral activity, and secondly to design drugs targeting particular proteins. In order to develop specific and efficient antiviral and antitumour drugs, a combination of biology, biochemistry, chemistry and structural biology is essential. The work presented here is aimed at defining the biological mechanisms and structural features of TIP30 and its protein partners, involved in cancers, Human Immunodeficiency Virus (HIV) and multiple sclerosis, and thus, providing the ground-work to help in the design of new molecules modulating TIP30 functions.

 

Native phasing

Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. I23 has been designed to provide an optimized environment for difficult S-SAD experiments, by increasing the anomalous signal with minimal noise.

Publications - +

Publications

Zhaoyang Sun, Kamel El Omari, Xiaoyu Sun, Serban L Ilca, Abhay Kotecha, David I Stuart, Minna M Poranen, Juha T Huiskonen. Double-stranded RNA virus outer shell assembly by bona fide domain-swapping. Nature Communications (2017).
Sina Wittmann, Max Renner, Beth R. Watts, Oliver Adams, Miles Huseyin, Carlo Baejen, Kamel El Omari, Cornelia Kilchert, Dong-Hyuk Heo, Tea Kecman, Patrick Cramer, Jonathan M. Grimes & Lidia Vasiljeva.The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA. Nature Communications (2017).
Oskar Aurelius, Ramona Duman, Kamel El Omari, Vitaliy Mykhaylyk, Armin Wagner. Long-wavelength macromolecular crystallography–First successful native SAD experiment close to the sulfur edge. NIMB (2016).
Thierry, Eric; Guilligay, Delphine; Kosinski, Jan; Bock, Thomas; Gaudon, Stephanie; Round, Adam; Pflug, Alexander; Hengrung, Narin; El Omari, Kamel; Baudin, Florence. Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains. Molecular cell 61 (1), 125-137, 2016.
Vendelboe, Trine V; Harris, Pernille; Zhao, Yuguang; Walter, Thomas S; Harlos, Karl; El Omari, Kamel; Christensen, Hans EM. The crystal structure of human dopamine β-hydroxylase at 2.9 Å resolution. Science Advances 2 (4), e1500980, 2016.      
Lye, Ming F; Sharma, Mayuri; El Omari, Kamel; Filman, David J; Schuermann, Jonathan P; Hogle, James M; Coen, Donald M.  Unexpected features and mechanism of heterodimer formation of a herpesvirus nuclear egress complex. The EMBO journal 34 (23), 2937-2952, 2015.
Hengrung, Narin; El Omari, Kamel; Martin, Itziar Serna; Vreede, Frank T; Cusack, Stephen; Rambo, Robert P; Vonrhein, Clemens; Bricogne, Gérard; Stuart, David I; Grimes, Jonathan M. Crystal structure of the RNA-dependent RNA polymerase from influenza C virus, Nature 527 (7576), 114-117, 2015.        
Zeev-Ben-Mordehai, Tzviya; Weberruß, Marion; Lorenz, Michael; Cheleski, Juliana; Hellberg, Teresa; Whittle, Cathy; El Omari, Kamel; Vasishtan, Daven; Dent, Kyle C; Harlos, Karl. Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling.      Cell reports 13 (12), 2645-2652, 2015.
Sewell, H; Tanaka, T; El Omari, Kamel; Mancini, EJ; Cruz, A; Fernandez-Fuentes, N; Chambers, J; Rabbitts, TH. Conformational flexibility of the oncogenic protein LMO2 primes the formation of the multi-protein transcription complex. Scientific reports 4, 2014.
El Omari, Kamel; Iourin, Oleg; Kadlec, Jan; Fearn, Richard; Hall, David R; Harlos, Karl; Grimes, Jonathan M; Stuart, David I.      Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1. Acta D 70 (8) 2197-2203, 2014.
El Omari, Kamel; Iourin, Oleg; Kadlec, Jan; Sutton, Geoff; Harlos, Karl; Grimes, Jonathan M; Stuart, David I. Unexpected structure for the N-terminal domain of hepatitis C virus envelope glycoprotein E1. Nature communications 5, 2014.              
Iourin, Oleg; Harlos, Karl; El Omari, Kamel; Lu, Weixian; Kadlec, Jan; Iqbal, Munir; Meier, Christoph; Palmer, Andrew; Jones, Ian; Thomas, Carole. Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus. Acta F 69 (1) 35-38, 2013.
El Omari, Kamel; Iourin, Oleg; Harlos, Karl; Grimes, Jonathan M; Stuart, David I. Structure of a pestivirus envelope glycoprotein E2 clarifies its role in cell entry. Cell reports 3 (1) 30-35, 2013.
Nichols, CE; Lamb, HK; Thompson, P; El Omari, Kamel; Lockyer, M; Charles, I; Hawkins, AR; Stammers, DK. Crystal structure of the dimer of two essential Salmonella typhimurium proteins, YgjD & YeaZ and calorimetric evidence for the formation of a ternary YgjD–YeaZ–YjeE complex. Protein Science 22 (5) 628-640, 2013.
El Omari, Kamel; Hoosdally, Sarah J; Tuladhar, Kapil; Karia, Dimple; Hall-Ponselé, Elisa; Platonova, Olga; Vyas, Paresh; Patient, Roger; Porcher, Catherine; Mancini, Erika J. Structural Basis for LMO2-Driven Recruitment of the SCL: E47 bHLH Heterodimer to Hematopoietic-Specific Transcriptional Targets. Cell reports 4 (1) 135-147, 2013.
El Omari, Kamel; Sutton, Geoff; Ravantti, Janne J; Zhang, Hanwen; Walter, Thomas S; Grimes, Jonathan M; Bamford, Dennis H; Stuart, David I; Mancini, Erika J. Plate tectonics of virus shell assembly and reorganization in phage φ8, a distant relative of mammalian reoviruses. Structure 21 (8) 1384-1395, 2013.            
El Omari, Kamel; Meier, Christoph; Kainov, Denis; Sutton, Geoff; Grimes, Jonathan M; Poranen, Minna M; Bamford, Dennis H; Tuma, Roman; Stuart, David I; Mancini, Erika J. Tracking in atomic detail the functional specializations in viral RecA helicases that occur during evolution. Nucleic acids research 41 (20) 9396-9410, 2013.
Bourhis, J-M; Mariano, N; Zhao, Y; Walter, TS; El Omari, Kamel; Delolme, F; Moali, C; Hulmes, DJS; Aghajari, N. Production and crystallization of the C-propeptide trimer from human procollagen III. Acta F 68 (10) 1209-1213, 2012.
El Omari, Kamel; De Mesmaeker, Julie; Karia, Dimple; Ginn, Helen; Bhattacharya, Shoumo; Mancini, Erika J. Structure of the DNA‐bound T‐box domain of human TBX1, a transcription factor associated with the DiGeorge syndrome. Proteins: Structure, Function, and Bioinformatics 80 (2) 655-660, 2012.
El Omari, Kamel; Hoosdally, Sarah J; Tuladhar, Kapil; Karia, Dimple; Vyas, Paresh; Patient, Roger; Porcher, Catherine; Mancini, Erika J. Structure of the leukemia oncogene LMO2: implications for the assembly of a hematopoietic transcription factor complex. Blood 117 (7) 2146-2156, 2011.
Stamp, Anna L; Owen, Paul; El Omari, Kamel; Lockyer, Michael; Lamb, Heather K; Charles, Ian G; Hawkins, Alastair R; Stammers, David K. Crystallographic and microcalorimetric analyses reveal the structural basis for high arginine specificity in the Salmonella enterica serovar Typhimurium periplasmic binding protein STM4351. Proteins: Structure, Function, and Bioinformatics 79 (7) 2352-2357, 2011.
El Omari, Kamel; Dhaliwal, B; Ren, J; Abrescia, NGA; Lockyer, M; Powell, KL; Hawkins, AR; Stammers, DK. Structures of respiratory syncytial virus nucleocapsid protein from two crystal forms: details of potential packing interactions in the native helical form. Acta F 67 (10) 1179-1183, 2011.
Stamp, Anna L; Owen, Paul; El Omari, Kamel; Nichols, Charles E; Lockyer, Michael; Lamb, Heather K; Charles, Ian G; Hawkins, Alastair R; Stammers, David K. Structural and functional characterization of Salmonella enterica serovar Typhimurium YcbL: an unusual Type II glyoxalase. Protein Science 19 (10) 1897-1905, 2010.
El Omari, Kamel; Porcher, Catherine; Mancini, Erika J. Purification, crystallization and preliminary X-ray analysis of a fusion of the LIM domains of LMO2 and the LID domain of Ldb1. Acta F 66 (11) 1466-1469, 2010.
El Omari, Kamel; Scott, K; Dhaliwal, B; Ren, J; Abrescia, NGA; Budworth, J; Lockyer, M; Powell, KL; Hawkins, AR; Stammers, DK. Crystallization and preliminary X-ray analysis of the human respiratory syncytial virus nucleocapsid protein. Acta F 64 (11) 1019-1023, 2008.
Szumska, Dorota; Pieles, Guido; Essalmani, Rachid; Bilski, Michal; Mesnard, Daniel; Kaur, Kulvinder; Franklyn, Angela; El Omari, Kamel; Jefferis, Joanna; Bentham, Jamie. VACTERL/caudal regression/Currarino syndrome-like malformations in mice with mutation in the proprotein convertase Pcsk5. Genes & development 22 (11) 1465-1477, 2008.
El Omari, Kamel; Stammers, David K. The design and development of drugs acting against the smallpox virus. Expert opinion on drug discovery 2 (9) 1263-1272, 2007.
El Omari, Kamel; Ren, Jingshan; Bird, Louise E; Bona, Marion K; Klarmann, George; LeGrice, Stuart FJ; Stammers, David K.      Molecular architecture and ligand recognition determinants for T4 RNA ligase. JBC 281 (3) 1573-1579, 2006.
El Omari, Kamel; Solaroli, Nicola; Karlsson, Anna; Balzarini, Jan; Stammers, David K. Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug design. BMC structural biology 6 (1) 1, 2006.
Balzarini, Jan; Liekens, Sandra; Solaroli, Nicola; El Omari Kamel; Stammers, David K; Karlsson, Anna. Engineering of a single conserved amino acid residue of herpes simplex virus type 1 thymidine kinase allows a predominant shift from pyrimidine to purine nucleoside phosphorylation. JBC 281 (28) 19273-19279, 2006.
El Omari, Kamel; Liekens, Sandra; Bird, Louise E; Balzarini, Jan; Stammers, David K. Mutations distal to the substrate site can affect varicella zoster virus thymidine kinase activity: implications for drug design. Molecular pharmacology 69 (6) 1891-1896, 2006.
El Omari, Kamel; Dhaliwal, Balvinder; Lockyer, Michael; Charles, Ian; Hawkins, Alastair R; Stammers, David K; Structure of Staphylococcus aureus guanylate monophosphate kinase. Acta F 62 (10) 949-953, 2006.
El Omari, Kamel; Bronckaers, Annelies; Liekens, Sandra; Pérez-Pérez, Maris-Jésus; Balzarini, Jan; Stammers, David K. Structural basis for non-competitive product inhibition in human thymidine phosphorylase: implications for drug design. Biochemical Journal 399 (2) 199-204, 2006.                   
El Omari, Kamel; Bird, Louise E; Nichols, Charles E; Ren, Jingshan; Stammers, David K. Crystal structure of CC3 (TIP30) implications for its role as a tumor suppressor. JBC 280 (18) 18229-18236, 2005.