The Electron Bio-Imaging Centre (eBIC) was established at Diamond following the award of a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC). The location of eBIC enables scientists to combine their techniques with many of the other cutting-edge approaches that Diamond offers; whilst a partnership with the University of Oxford allows users to access the Polara, a high-containment cryo-electron microscope.
eBIC provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for both single particle analysis and cryo-tomography. Currently eBIC has two Titan Krios microscopes whereas the Polara microscope is located at the University of Oxford. For more information on eBIC please follow this link.
Titan Krios I and II are state of the art 300 KeV cryo-electron microcopes from FEI. They are equiped with the latest generation of direct electron detectors, the FEI Falcon II and the Gatan Bio-quantum K2 summit. Both detectors are intergated into FEI's automated data aquisition softwares for single particle and tomography, EPU and TOMO4 respectively.
The Polara is a 300 KeV cryo-electron microscope from FEI. It is also equipped with a Gatan Bio-quantum K2 summit detector. This detector can be used for semi-automated data collection with SerialEM for both single particle and tomgraphy aplications. The Polara is operable at containment level 3.
At the microscope
Users of both Krios 1 (m02) or Krios 2 (m03) should meet their local contact at Krios 1 as grid loading is carried out for both microscopes in lab. 7 of zone 1. The standard user day is listed below:
- 9 - 9.30: Session starts
- 9.15 - 10.15: Load up to 8 grids into auto grid cartridges and the Krios cassette
- 10.20 - 10-30: Load auto grid cassette into the Krios
- 10.30 – 12: Survey grids to determine the best grid
- 12 – 12.45: Collect grid atlas image
- 12.45 – 3: Setup EPU/TOMO4, microscope alignments, detector gain references
- 3 – 4: Check images, assess CTF and particle density.
- Automatic data collection for ~40 hours.
EBIC staff will check data acquisition is proceeding from home but the user should also monitor their data collection and email their local contact if they observe issues.
If you have written your data to USB HDDs please make sure that your local contact has the correct mailing address for return postage. Alternatively FTP/Globus may be used to transfer your data from Diamond to your home institution.
Users publishing work containing data from eBIC should ensure they:
- Acknowledge DLS and any of its personnel in any published material that results from their use of the Cryo-EM facilities. The following statement should always be included in any publications:
“(We acknowledge) Diamond for access and support of the Cryo-EM facilities at the UK national electron bio-imaging centre (eBIC), proposal EM####, funded by the Wellcome Trust, MRC and BBSRC.”
- Notify DLS of the title, authors, facility, journal and reference number, date of acceptance and publication of any scientific publications or papers as a result of their use of the beamtime
- Notify DLS if they plan to publish any materials e.g. general reports, press releases, etc. that result from their use of the beamtime prior to the material being published and allow time for DLS to review such material and give its prior permission to publish such material if reasonably requested by DLS.