About eBIC

The Electron Bio-Imaging Centre (eBIC) was established at Diamond following the award of a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC). The location of eBIC enables scientists to combine their techniques with many of the other cutting-edge approaches that Diamond offers; whilst a partnership with the University of Oxford allows users to access the Polara, a high-containment cryo-electron microscope.

eBIC provides scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for both single particle analysis and cryo-tomography. Currently eBIC houses four Titan Krios microscopes, a Talos Arctica and a Scios cryo-FIB/SEM. The Polara microscope is located at the University of Oxford.

Call for cryoFIB-SEM applications

The Scios™ DualBeam™ Scanning Electron Microscope (SEM) and Focused Ion Beam (FIB) system.
The Scios™ DualBeam™ Scanning Electron Microscope (SEM) and Focused Ion Beam (FIB) system.

The cryo focused ion beam scanning electron microscope at Diamond Light Source is currently in its commissioning period and we would like to announce a call for user proposals. This first commissioning call will provide time for a limited number of peer reviewed user proposals (our expectations are that no more than 2 or 3 proposals will be accepted in this first commission call). 

Initially we are offering only milling of wedges and lamella at thicknesses around 200-300nm from cells grown on EM grids for subsequent cryoEM/ET imaging. (We will not be offering a fluorescence correlative workflow or cryo lift out at this stage). 
Interested potential Diamond users should log in and submit their proposal, or as a new user simply register in our User Administration Software (UAS) - https://uas.diamond.ac.uk/uas/#register. Once registered or logged into the UAS you should choose the “commissioning” access route and the instrument M05.  
All proposals will be assessed and equipment time will be granted based on scientific merit with a strong emphasis on technical feasibility and sample suitability. Applicants should include evidence that their samples are well vitrified and that the EM grid support films are intact after clipping into FEI autogrid rims. All grids must be pre-screened in an autoloader system and pass at least the following but not exclusive set of criteria: appropriate ice thickness, majority of the EM grid support film being intact, and good sample concentration. 
We strongly advise that you contact eBIC staff for further details and advice. For technical questions about proposed experiments please contact corey.hecksel@diamond.ac.uk.
 
 

First session taken by a BAG super-user in eBIC's new control room

First BAG super-user led session from the University of Oxford.
First BAG super-user led session from the University of Oxford.

In February 2018 work was completed on a new control room for remote control access to eBIC's four Krios microscopes. 

The new control room facilitates a more efficient way of working with the vast majority of eBIC activities able to be undertaken in the same building.  It also enables users to enjoy a more comfortable working environment when visiting eBIC. 
 
On 9th February 2018 the first candidates successfully completed BAG super-user accreditation, and are now able to enjoy greater flexibility during their user sessions. 

 

  • The eBIC team
  • The eBIC executive committee outside the building.
  • Alex Buzduga and Alistair Siebert with Krios I at Diamond.
  • A cryo EM map of Tobacco Mosaic Virus, with the atomic structure (colours) built into it.
  • Detail of the Adeno virus.
1 2 3 4 5
19/02/2018:

eBIC control room now fully operational for remote control access to all Krios microscopes.


Where: eBIC
Who: eBIC
09/02/2018:

First BAG super user accreditation/training successfully completed.

01/01/2018:

eBIC welcomes three new members of staff, Katie Cunnea (Centre coordinator) James Gilchrist (Support Scientist) and Zhengyi Yang (Postdoc).

14/07/2017:

CryoEM Sample Training workshop - 2nd - 4th October 

Open for applicaitions 

24/10/2016:

eBIC Director announced: Introducing Prof Peijun Zhang

27/06/2016:

Krios II welcomes its first users from the University of Oxford

29/06/2015:

Krios I welcomes first users from MRC LMB Cambridge

21/01/2015:

[Publication] A national facility for biological cryo-electron microscopy. Saibil, H. R., Grunewald, K. & Stuart, D. I. (2015). Acta Cryst. D 71, 127-135. DOI: 10.1107/S1399004714025280