Wes Armour, Diamond
There has been much interest in recent years regrading the viability of performing X-ray microtomography experiments on MX beamlines.
This has been motivated by several different problems that arise in certain MX experiments. For example advancements in crystallisation techniques such as the use of Lipidic cubic phases for the crystallisation of membrane proteins result in crystals forming in optically opaque liquids. For diffraction experiments performed on samples in low symmetry spacegroups or at longer wavelengths a method of correcting for absorption effects is important. To do this without using the diffraction data collected, knowledge of the sample shape is required.
In this session I will discuss the experimental method used to perform X-ray microtomography experiments at diamond MX beamlines. I will then cover the subsequent data analysis and present a software package called Aberration, which is designed to automatically correct, realign, reconstruct and segment X-ray microtomography data collected on MX beamlines. I will then discuss how this data is used by a software package called Absolve to produce an absorption correction for collected diffraction data. Finally I will give an overview of how this might be integrated into beamline systems.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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