Latest News
Since reopening in July to users after the end-station upgrade further investigations into the use of compound refractive lenses for delivering beam have been undertaken. Latest results now allow us to open up the available energy range to cover 6.5 to 17 keV, providing beamsizes of 8.0 x 2.0 through to 193 x 193 µm. See below or contact a member of the I04 team for more details.
Beamline Features
I04 is one of three closely related (I02 and I03) macromolecular crystallography beamlines for interpreting the structure of complex biological samples. The typical working wavelength is 0.9795 Å (12.658 keV) but it is tuneable over the wavelength ranges of 0.73 - 1.91Å. The recent end-station upgrade has enabled new and improved beamline features which include:
- 20 x 20 µm beam at Se K edge delivering 2 x 1011 ph/s at the Se K edge
- Minibeam apertures (10 or 20 µm). In development - please ask in advance of visit if you wish to use.
- Microfocus beam at discrete energies (8.0 x 2.0 µm at 13.1keV and 7.15keV)
- High resolution viewing system
- Accurate sample centering stages
- Simple beam visualisation procedures
- Finer attenuation control
- Rigaku ACTOR sample changer
- ADSC Q315r detector
- Vortex fluorescence detector
- Automated data processing
- Possibility to focus at detector (energy dependent function)
- Remote access
I04 Contacts
| Principal Beamline Scientist: Dave Hall Tel: +44 (0) 1235 778926 E-mail: Dave.Hall@diamond.ac.uk | Senior Beamline Scientist: Ralf Flaig Tel: +44 (0) 1235 778412 E-mail: Ralf.Flaig@diamond.ac.uk |
| Support Scientist: | PDRA: TBA |
| Beamline number: 01235 778708 | |
I04 Current Specifications
| Beamline I04 | |
| Standard energy (Å / keV) | 0.98 / 12.66 |
| Flux at 12.66 keV (ph/s) | 2.0 x 1011 |
| Beam size at 12.66 keV (µm) | 20 x 20 |
| Wavelength range (Å) | 0.73 - 1.91 |
| Energy range (keV) | 6.5 - 17.0 |
| Beam size at sample (range, µm)* | 8.0 x 2.0 - 193 x 193* |
| Detector | ADSC Q315r |
| Time to collect 180o data set (minutes) | 7.5 + |
| Sample Changer | ACTOR |
| No. of pins in dewar | 80 |
| Sample exchange time (seconds) | 30 |
| Guide to samples screened/hour (user centering) | 30 |
* see below for description of capability to vary beam size on I04
+ 1o per 1 second exposure 180 frames
I04 Beamsize
The upgrade of the I04 end-station provides for variable beam focus at the sample using compound refractive lenses which is energy/wavelength dependent. At the Se K edge a focus of 20 x 20 µm is achieved with a flux of 2.0 x 1011 photons/sec. Depending on the energy this beam focus can be varied from a minimum of 8 x 2 µm (h x v) through to 190 x 190 µm. The beamline is currently set to deliver the smallest beam size at both 7.15 and 13.1keV.
Note that in the case of SeMet Mad experiments, at the Se K edge the beam size is ~ 20 x 20 µm and choosing 13.45keV for your high energy remote data set will give a similar beamsize. Furthermore 10 and 20 µm apertures are available for delivering smaller beams to your crystal when using the larger beam sizes. The figure below illustrates the variation of beamsize over the available energy range.
At the smallest focal beam size this gives a flux density of 1 x 1016 photons/mm2/second and is available with the usual automation facilities expected of the phase I beamlines.