Please see the current status tab for a detailed breakdown of the status of the beamline
I24 is a tuneable microfocus beamline for MX. It was the first beamline if its kind to be built in Europe and has been operating since 2008. It is currently in the process of a complete rebuild. The rebuild of the endstation is complete (hardware-wise) and new mirrors are now at Diamond and will be installed early 2016. When complete we will be able to offer a ~2.5x5 micron beam with a state-of-the-art endstation.
Please note that in situ data collection is now available. For the first few weeks of operation we would like to offer this on weekdays (only) in case of problems. Please get in touch with your local contact or Robin if you would like to try it.
The beamline offers extremely high flux densities with the ability to investigate virus structure, membrane proteins, microcrystals (crystals as small as 1.5 microns have been measured successfully). It combines versatile optics and the most sophisticated detectors available with the most advanced automation systems to enable sample location, data collection and analysis.
Please email us detail of any problems you run into, or any other comments or suggestions.
Data processing - vertical goniometer
Please remember to take the new rotation axis into account when processing your data. In XDS use
ROTATION_AXIS= 0 -1 0
Sample input files in /dls_sw/i24/scripts/data_processing/
For mosflm, make sure to use version 7.1.3 (this version is installed at Diamond). Detailed instructions can be found here.
(Very) briefly. Start imosflm
> imosflm --expert
AFTER (and only after) reading the images, select the "Experiment settings" option from the "Settings" menu. Because the "expert" option has been selected there is now a third tab labelled "Developers" - select this tab. In this window, select a value of 270 from the drop-down menu for the entry "Detector omega:" (the initial value will be 0).
Ask your local contact if you need a hand, or if you have any questions.
Automatic processing should be working. Let us know if you run into problems
New remote access connection available
Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.
New web interface for ISPyB launched. For more information click here.
Deadline for Call for Proposals for AP16 is 25th March 2014. See here.
Fast grid scans available up to 100Hz
Loan pucks available upon request, check here
Test your network connection to Diamond with our SpeedTest
Updated excel spreadsheet for shipments in ISPyB. Download and use the latest version.
New Pilatus 3 detector available. Run even faster!
101 structures from I24 have been deposited in the PDB!
I24 reopen to users on 1st May.
New robot update: faster exchange time and improved recovery procedure
Remote access available on all MX beamlines at all times since January 2013
|Detector type||Pilatus3 6M (100 Hz max. frame rate).|
|Available X-ray wavelengths / energy (Å)||0.7 - 2.0|
|Available wavelengths (keV)||6.4 - 20.0|
|Maximum attainable resolution (Å)||0.8|
|Flux Details||Into full beam of 10x10µm @ 12800 eV and 300 mA ring current|
|Absorption edge scans||Optimised SAD and MAD experiments|
|Minimum focused beam size (µm)||10 x 10 (FWHM)|
|Maximum focused beam size (µm)||50 x 40 (FWHM)|
|Minimum beam size (µm) (cutting secondary source)||5 x 5 (FWHM) with 1x10^11 ph/s|
|Sample changer||Irelec CATS robot with unipucks - exchange time ~ 45 sec - 9 pucks in dewar, 16 pins per puck (total 144 pins)|
|Pins and Pucks||SPINE standard pins and Unipucks|
Humidity Controller (HC1) for RT data collection
|Available on request|
|Spectroscopy||Available on request|
Please discuss your requirements with your local contact or a member of the beamline team before your experiment.
Shift timings: unless explicitly arranged with beamline staff, the start and end times of all (academic, industrial and in-house) shifts are as below:
NB: short shifts run to different timings. If you have a 'short' shift you will be emailed details of when the shift starts/ends.
Diamond Light Source is the UK's national synchrotron science facility, located at the Harwell Science and Innovation Campus in Oxfordshire.
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