Diamond Annual Review 2023/24

8 D I A M O N D L I G H T S O U R C E A N N U A L R E V I E W 2 0 2 3 / 2 4 Microscope Main Capabilities Accelerating Voltages Operational Status Titan Krios I Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2015 Titan Krios II Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2016 Titan Krios III Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2017 Titan Krios IV Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2017 Titan Krios V (Industrial) Cryo-EM, Cryo-ET 80, 120, 200, 300 kV Operational since 2018 Glacios (Industrial) Cryo-EM, Cryo-ET 200 kV Operational since 2019 Aquilos 2 Cryo-SEM, Cryo-FIB 3 to 30 kV Operational since 2020 Leica cryo-CLEM Cryo-CLEM N/A Optimisation JEOL ARM200F Atomic scale STEM imaging, EELS, EDX, electron diffraction 80, 200 kV Operational since 2017 JEOL ARM300F Atomic scale TEM and STEM imaging, electron diffraction, 4D-STEM, EDX 30, 60, 80, 160, 200, 300 kV Operational since 2017 JEOL Ion Beam 4700F SEM, FIB 1 to 30 kV Operational since 2020 Electron Microscopes Beamline Development and Technical Summary I n its seventeenth year of experiments, Diamond is now operating with 33 beamlines and eight electron microscopes dedicated for experiments. A further five instruments are available for experiment support and sample preparation. Ten of the instruments specialise in life sciences and make up eBIC (electron Bio-Imaging Centre), with two provided for industry use in partnership with Thermo Fisher Scientific. Two of the electron microscopes are dedicated to advanced materials research and are supplied by Johnson Matthey and the University of Oxford. These, along with a further instrument for sample preparation, form ePSIC (electron Physical Science Imaging Centre) and are operated under strategic collaboration agreements to provide for substantial dedicated peer reviewed user access. Both eBIC and ePSIC are next to the Hard X-ray Nanoprobe beamline (I14). Along with eBIC and ePSIC, the UK X-ray Free Electron Laser (XFEL) Hub, the Membrane Protein Laboratory (MPL), the XChem fragrment screening facility, the LabSAXS facility and the ActiveMaterials Laboratorymake up the complementary integrated facilities available at Diamond. For academic research, Diamond instruments (beamlines and microscopes) are free at the point of access through peer review. For proprietary research, access can be secured through Diamond’s industry team. The instruments and beamlines are organised into eight science groups as described below. Krios I Krios IV Krios V Glacios Aquilos ARM 200F ARM 300F Talos Arctica Scios Krios II Krios III Glacios2 Cryo- CLEM Ion Beam 4700F I04 Microfocus MX I05 ARPES: Angle-Resolved Photoemission Spectroscopy I06 Nanoscience I07 Surface and Interface Di raction B07 VerSoX: Versatile Soft X-ray I08 Scanning X-ray Microscopy I09 Atomic and Electronic Structure of Surfaces and Interfaces I10 BLADE: X-ray Dichroism and Scattering I11 High Resolution Powder Di raction & Long Duration Experiments (LDE) I12 JEEP: Joint Engineering, Environmental and Processing DIAD: Dual Imaging and Di raction I13 X-ray Imaging and Coherence Hard X-ray Nanoprobe I14 XPDF: X-ray Pair Distribution Function I15-1 Materials and Magnetism I16 B16 Microfocus Spectroscopy I18 Core XAS: X-ray Absorption Spectroscopy B18 Active Materials Laboratory Small-Molecule Single-Crystal Di raction I19 LOLA: Versatile X-ray Spectroscopy I20 Inelastic X-ray Scattering I21 High Throughput SAXS B21 Small Angle Scattering and Di raction I22 MIRIAM: Infrared Microspectroscopy B22 LongWavelength MX I23 Circular Dichroism B23 Microfocus and Serial MX I24 I02-2 VMXi: Versatile MX in situ I02-1 VMXm: Versatile MX micro Cryo-TXM: Cryo-Transmission X-ray Microscopy B24 Optics and Metrology Laboratory I03 MX I04-1 MX Extreme Conditions I15 XChem facility for fragment-based screening UK X-ray Free Electron Laser (XFEL) Hub Electron Physical Science Imaging Centre (ePSIC) Electron Bio-Imaging Centre (eBIC) Test Beamline O ine SAXS Membrane Protein Laboratory Macromolecular Crystallography Soft Condensed Matter Biological Cryo-Imaging Spectroscopy Magnetic Materials Crystallography Imaging and Microscopy Structures and Surfaces