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The B24 beamline is designed to operate at low (soft) X-ray energies in the region termed the “water window” although it also has the capability of operating at higher energies.
The water window is the region between the K-absorption edges of carbon at 284 eV and oxygen at 543 eV. Between these two energies oxygen is virtually transparent to X-rays whereas carbon is highly absorbing. Thus there exists a natural contrast between the vitreous ice surrounding a frozen biological cell and the carbon containing structures making up the cell.
For further details on B24, take a look at our beamline pages.
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